RECOMBINANT DNA TECHNOLOGY * It is a technology that allows DNA to be produced via artificial means. * It is the joining together of DNA molecules from two different species that are inserted into a host organism to produce new genetic combinations that are of value to science‚ medicine‚ agriculture‚ and industry. * Since the focus of all genetics is the gene‚ the fundamental goal of laboratory geneticists is to isolate‚ characterize‚ and manipulate genes. Although it is relatively
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DNA Extraction from Wheat Germ and Making an Agarose Gel AIM: To be able to make and agarose gel and perform gel electrophoresis in six different dyes. Also‚ to extract DNA from wheat germ. INTRODUCTION: Agarose gel is a substance that is used in science for gel electrophoresis and size exclusion chromatography. These processes use agarose gel to separate and analyze proteins and DNA. The medium is composed of a purified agarose powder that has been boiled in a buffer solution and then cooled
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DNA Extraction of a Musa acuminata Biology Lab Report \Analyze and Interpret A number of steps are required to isolate DNA from cellular contents. Describe what happens at each step‚ and why it acts to separate the parts of the cell. /6 There are three specific steps required to isolate DNA from its cellular contents. The steps used to remove and expose DNA from its cell are: breaking down the food type you are using by crushing it‚ for example a banana or strawberries‚ exposing the substance
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Strawberry DNA lab Extraction Purpose: The purpose of this lab experiment is to see if this particular type of fruit which in this case is a strawberry contains DNA. Using materials such as a Ziploc bag to help keep the entire strawberry in one place while it gets smashed and test tube to help better see the DNA and break down of the liquid in the left over strawberry sediments. Background information: The long thick fibers of DNA store the information for the function of the chemistry
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DNA Technology: Biotechnology 1) Selective breeding a. 2) Genetic engineering – humans tinker with organisms genes a. Cloning –take haploid cel and replace with a diploid nucleus (comes from organism whos traits you want to duplicate. b. Recombinant DNA – DNA from 2 or more sources. Done by Euk cells during Meiosis. Always from same molecule. 3) Biologists first started doing recombinant DNA from a prok cell and combined it with another prok cell because it was easier. 4) DNA from a
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placed in an electrophoresis machine. An electric current is run through the machine and the different sized molecules form bands on the gel matrix. In visualization‚ the gel is dyed so the results become present. This is used in modern crime labs‚ figuring out DNA‚ which plays a key role in many criminal trials. The researcher completed this experiment to figure out who committed the murder using gel electrophoresis. The researcher followed five steps in this experiment; First was placing the gel in
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BIOTECHNOLOGY RECOMBINANT DNA technology 1. This is a modern biotechnological advance ‚ in which a desired gene fragment can be inserted in to a cloning vector and the resulting DNA (Recombinant DNA) can be amplified in suitable host. 2. A vector can be a plasmid‚ cosmid‚bacterophage‚retroviruses‚ animal and plant viruses or artificial chromosomes like YAC‚ BAC‚or HAC.(Yeast artificial chromosome‚ bacterial........) 3. The rec. DNA produced can be amplified or cloned in a suitable vector
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Objective: The purpose of this lab is to allow you to become familiar with a procedure for extracting DNA‚ collecting DNA samples‚ and to observe the physical characteristics of DNA Background: The wild strawberry is a diploid because it only has two sets of chromosomes. While the grocery store kind is an octoploidy because it has eight sets of chromosomes. The reason we chose the grocery store strawberry is because we’d be able to extract more DNA. Ripe strawberries make enzymes which help break
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wberry DNA Lab ReportMeredith Anderson Mrs. Berman Biology October 17‚ 2012 Extracting DNA from Strawberries Problem/Question: Successfully extract the DNA from strawberries. Theory/Hypothesis: N/A Variables: Independent – strawberries Dependant – the DNA Controlled – 450 ml of water‚ 50 ml of soap‚ 1 tsp of salt‚ 5 ml isopropyl alcohol Apparatus and Materials: N/A Procedure and Control of Variables: N/A Data Collected: See attached paper. Data Processing and Presentation:
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DNA Extraction Lab Problem Statement: Do you think you have ever eaten DNA? Background Information: DNA is too small to see under a regular microscope‚ so how can it be studied? DNA is a large molecule found in all living things; therefore it is possible to extract it from cells or tissues. All we need to do is disrupt the cell’s plasma membrane and nuclear envelope‚ make the DNA clump together and - voila! - DNA extraction is possible. DNA extractions from onion‚ bananas‚ liver‚ or wheat
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