Introductory Biochemical Laboratory Worksheet 10 - DNA Melting Curve Course Code: BICH172 Course Name: Introductory Biochemical Laboratory Student Name: CHUNG YUK MAN EMILY Student ID: 10198465 Group Number: 10 Date of Experiment: 16th November 2010 Q1. State the experimental aim and describe the term “hyperchromic effect”. (2 mark) The experimental aims are to study the basic structural characteristic of DNA molecules through UV spectrum and thermal denaturation
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DNA Extraction from Fruit 1. What was the purpose of adding liquid soap and salt in step #1 and how does NaCl contribute to maximum DNA extraction. The purpose of using soap was to destroy the membranes inside a kiwi cell. Soap helped with that because it dissolves the membranes easily. Salt or NaCl was used to remove proteins and carbohydrates. NaCl caused the proteins and carbohydrates to precipitate. 2. Why was it necessary to “mush” the kiwi by hand? If the step was omitted‚ what
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Importance of DNA Typing i Outline Thesis Statement: Although having an infant’s DNA typing done at birth can effectively analyze important health information‚ it should be an individual’s given right to accept and/or decline the storage of their private information. I. Some individuals are unaware of DNA typing of infants at birth. A. An individual may be described as parent‚ or person distinguished from others by a special quality. B. Some individuals claim that DNA typing
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BIOTECHNOLOGY RECOMBINANT DNA technology 1. This is a modern biotechnological advance ‚ in which a desired gene fragment can be inserted in to a cloning vector and the resulting DNA (Recombinant DNA) can be amplified in suitable host. 2. A vector can be a plasmid‚ cosmid‚bacterophage‚retroviruses‚ animal and plant viruses or artificial chromosomes like YAC‚ BAC‚or HAC.(Yeast artificial chromosome‚ bacterial........) 3. The rec. DNA produced can be amplified or cloned in a suitable vector
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DNA and Its Wonders (Each Title corresponds with a picture on the Glog) DNA in Society Genetic Testing – Genetic Testing allows the diagnosis of how vulnerable an individual is to inherit the disease. The results of a genetic test can confirm a suspected genetic condition or help determine a person’s chance to develop or pass on a genetic disorder. Being able to have this information is a great preliminary defense. Crime Scene Investigation – As the world progresses with new innovations we
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Conclusions of DH5α DNA transformation with red colonies resistance to ampicillin and the lacZ gene Introduction: In this experiment‚ a plasmid with a gene that has resistance to the antibiotic ampicillin and has lacZ is used to transfer the resistance into E. coli bacteria in red colonies. This same technique is used to give diabetics their insulin‚ and to give dwarfs growth hormones. The point of this lab is to give the groups an idea how DNA can be transformed
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The Discovery of the Structure of DNA Determining the structure for DNA was one of the outstanding science achievements of the 20th century. It started in the late 1940’s‚ when scientists were aware that DNA was most likely the molecule of life‚ even though many were sceptical since it was so "simple." They also knew that DNA included different amounts of the four bases: adenine‚ thymine‚ guanine and cytosine (usually abbreviated A‚ T‚ G and C)‚ but nobody knew what the molecule might look like
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Comparison chart DNA RNA Stands for: DeoxyriboNucleicAcid RiboNucleicAcid Definition: A nucleic acid that contains the genetic instructions used in the development and functioning of all modern living organisms (scientists believe that RNA may have been the main genetic material in primitive life forms). A single-stranded chain of alternating phosphate and ribose units with the bases Adenine‚ Guanine‚ Cytosine‚ and Uracil bonded to the ribose. RNA molecules are involved in protein synthesis
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Ka Zhu The Importance of the Structure of DNA Understanding ideas at a macroscopic scale is simple. Looking at a clock‚ observing and understanding the movements of the hands over the numbered surface are‚ in essence‚ all one requires to use the device. In order for innovation to occur‚ it is imperative to understand the inner workings of the device on a microscopic scale to modulate its properties. Such is the case for many innovations in science‚ from the heat engine to penicillin‚ and is no
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Who Discovered DNA? Heather Kane The discovery of deoxyribonucleic acid‚ more commonly known as DNA‚ has been the foundation for much scientific work. This fundamental discovery was credited to James Watson and Francis Crick. Many people believe that another person‚ Rosalind Franklin‚ also played a large role in the research. How much did she contribute to the discovery? Why is her name left unrecognized? This paper will discuss her part in the search and whether her name should appear next to
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