Discussion Using the Thin Layer Chromatography experiment‚ caffeine was found to be the most polar due to the fact that it stayed closer to the stationary phase. Caffeine contains four amine groups that are extremely polar as a result of the hydrogen bond and amide functional group. Acetaminophen was found to be the second most polar analgesic drug tested. Acetaminophen contains a polar alcohol group on one side and amide group on the other but also includes non-polar functional groups that consisted
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Separation techniques LIQUID CHROMATOGRAPHY ‘THE ART OF SEPARATION’ CHROMATOGRAPHY – AN INTRODUCTION Chromatography is a technique through which a mixture of chemical components are separated‚ identified and determined accurately. This technique while provides a way for analytical separations‚ also useful for preparative techniques by which pure compounds can be obtained. Detector Signal Blue Compound Sample Injection + Mobile Phase Retention Time Red Compound It is i defined d fi d as a
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Performance Liquid Chromatography (HPLC) Instructor: Dr. Hüseyin BOZKURT High Performance Liquid Chromatography (HPLC) is one mode of chromatography‚ the most widely used analytical technique. Chromatographic processes can be defined as separation techniques involving mass-transfer between stationary and mobile phases. Present day liquid chromatography that generally utilizes very small packing particles and a relatively high pressure is referred to as high performance liquid chromatography (HPLC).
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Question 1 1.1 Silica gel chromatography: This is known as the stationary phase in column chromatography. Firstly‚ the tapered exit of the column is sealed using porous material. This porous material serves as support for the packing material‚ and prevents it from exiting the pipe. Thereafter‚ silica gel is compacted into the glass pipe to make the separating column. In finishing preparation of the column‚ the solvent which is used as the mobile phase is then passed through the dry column. Then the
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Expt. 8.1 Thin Layer Chromatography (TLC) Analysis of Analgesics Krista Jinks CH 211L Section F 10/27/14 I. Abstract: The objective of this experiment was to analyze analgesic tablets by TLC to determine what analgesics they contain. The analgesics that could be in the tablets included ibuprofen‚ acetaminophen‚ aspirin‚ and caffeine. Through the use of TLC‚ unknown substance #62 was found to be Bayer Aspirin because it only contained aspirin. II. Introduction: 1. Briefly describe
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Abstract Pigments extracted from different greens have different polarities and may be different colors. Mixed pigments can be separated using chromatography paper. Chromatography paper is able to separate mixed pigments due to their polarity and solubility. Pigments of chlorophyll a‚ chlorophyll b and beta carotene will be separated on chromatography paper because each has its own polarity and solubility‚ which results in different distance traveled up the paper. Beta carotene is non-polar so it
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References: 1) E. Stahl‚ Thin Layer Chromatography‚ Springer-Verlag‚ New York‚ 1969 2) Camag Scientific‚ Inc.‚ 515 Cornelius Harnett Drive‚ Wilmington‚ NC 28401‚ phone 800-3343909‚ fax 919-343-1834 3) Desaga GmbH‚ P.O. Box 101969‚ D-69009 Heidelberg‚ Germany‚ phone 0-62-21-83590‚ fax 0-62-21-840887
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Resolution of Matter into Pure Substances – Paper Chromatography Abstract The purpose of this laboratory was to determine how different substances have different solubilities in a given solvent. This was accomplished using the method of chromatography. The solubility of each substance was first determined by dropping a drop or two of each on a line drawn 2 inches above the bottom of a piece of paper. Then the paper was lightly bent in order to tape both sides of the paper leaving about a 4 centimeter
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Column and Thin Layer Chromatography: The Separation of Spinach Pigment Abstract Spinach extract was separated into fractions containing compounds of similar polarity by column chromatography. Based on solid-liquid phase partitioning‚ this separation technique exploited the different polarity of the compounds in the spinach extract. Three fractions with different colors were obtained. The extract and its fractions were analyzed using thin layer chromatography (TLC). The TLC results showed that
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Full Report on Exercise 4.2 ESTIMATION OF PROTEIN CONCENTRATION BY SPECTROPHOTOMETRY And Exercise 4.3 GEL FILTRATION CHROMATOGRAPHY Joel Don M. Untalan CHEM 160.1 – 1L AY 2013 – 2014 Groupmates: Sonette Yao Kristopher Quilan Laboratory Instructor Carmelo C. Briones I. Introduction Analyzing proteins in determination of protein concentration by spectrophotometry is important. It determines to what concentration of a certain protein is in a crude sample. In this technique‚ a wide
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