Liquid Chromatography Purpose: The purpose of this lab was to separate substances based on their polarity by using liquid chromatography. Data Table: Red Dye Blue Dye Run#1 Run#2 Run#3 Run#1 Run#2 Run#3 Start of Band(mL) 1.50 2.20 1.00 2.70 3.00 2.00 End of Band(mL) 2.70 3.00 2.00 6.40 5.50 6.00 Beaker Eluant Observations 1 H2O White powder 2 5%isopropyl Red powder 3 28%isopropyl Blue powder 4 70%isopropyl Oily residual Calculations: W = Vend – Vstart
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The History of Paint Pigments Chem 111-404 April 24‚ 2013 Introduction: This report expresses five different pigments that were formed during chemical reactions. Three out of the total five reactions went through a metathesis‚ or double-replacment reaction. A metathesis reaction can be defined as “a chemical reaction in which an element or radical in one compound exchanges places with another element or radical in another compound.” (Webster). The other two pigments underwent a neutralization
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Chloroplast Chloroplasts are the food producers of the cell. This organelle is only found eukaryotic cells. They are only found in plant cells. Chloroplasts contain small pigments called chlorophylls. Animal cells do not have chloroplasts. All green plants you see are working to convert the energy of the sun into sugars. Plants are the basis of all life on Earth. They create sugars‚ and the oxygen that we breathe. This process is also known as chloroplast. The mitochondria work in the opposite
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Problem: Why do leaves change color in the fall? Background: Leaves contain many pigments such as chlorophyll b‚ xanthophyll‚ and beta carotene besides chlorophyll a. They are part of the photosystem in the thylakoids. Pigment molecules can be separated by a procedure called chromatography. The procedure uses a special paper and solvent. The chlorophyll molecules adhere to the paper. The solvent molecules move up the paper by capillary action. Each chlorophyll molecule will travel up the paper at
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Chloroplasts are organelles present in plant cells and some eukaryotic organisms. Chloroplasts are the most important plastids found in plant cells. It is the structure in a green plant cell in which photosynthesis occurs. Chloroplast is one of the three types of plastids. The chloroplasts take part in the process of photosynthesis and it is of great biological importance. Animal cells do not have chloroplasts. All green plant take part in the process of photosynthesis which converts energy into
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Paper chromatography Presented by – Miss. Shruti Vilas Kharat. SYBSc / FS13164 History- Paper Chromatography (PC) was first introduced by German scientist Christian Friedrich Schonbein (1865). PC is considered to be the simplest and most widely used of the chromatographic techniques because of its applicability to isolation‚ identification and quantitative determination of organic and inorganic compounds. Definition- Paper chromatography is an analytical method technique for separating and
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Column Chromatography of Plant Pigments Paul Ibarbia‚ Gene Paolo Jasmin‚ Gianpaolo Jimenez and Lorenzo Labicane* Department of Biology‚ University of Santo Tomas‚ Manila‚ Philippines Abstract Column chromatography of plant pigments is the separation of plant pigments extracted from Capsicum frutescens (siling labuyo). No slurry is prepared of the eluent; eluent with the stationary phase is set and then carefully poured into the column. The eluent are the solvents which are responsible for the
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Chloroplasts Chloroplasts are food producers of the cell and they make the plant green. They are organelles found in plant cells and animal cells do not have chloroplasts. Also‚ they have a eukaryotic alga that conducts photosynthesis. In a plant cell there can be anywhere from 10 to 100 chloroplasts in one cell. They are approximately 4 to 6 cm in diameter and shaped like a satellite dish with the concave face forward toward the light. Also‚ they are similar to mitochondria but mitochondria works
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Light Reactions in Isolated Chloroplasts Racquel Currie University of Minnesota Minneapolis‚ Minnesota Hypothesis If isolated chloroplasts are boiled‚ the enzymes that occur in photosynthesis are disrupted and the DCIP will not reduce. Chloroplasts are a type of plastid found within the plant they are the basis of photosynthesis. Without photosynthesis there would be no life on earth. Photosynthesis takes the energy from sunlight and coverts it into a chemical energy that can be used by
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EXPERIMENT 5: CHROMATOGRAPHY Abstract Paper Chromatography uses a chromatogram paper as its stationary phase and the solvent as the mobile phase. Retention factor is the ratio of the distance travelled by the sample to the distance travelled by the solvent. This experiment aims to separate organic compounds‚ to compute Rf values and to identify unknown compounds using Rf values. The ten samples underwent paper chromatography to determine the components of the unknown sample. The mobile phase allowed
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