The purpose of this experiment was to test how effective certain homogenates were as buffers. Buffers are devices that keep pH within maintainable boundaries so something can function. When something is too basic (has too much OH-) the buffer adds H+ and vice versa in order to create water to keep the pH at an acceptable range. Each group (I was with William Yung for this experiment) was tasked with testing one homogenate. The homogenate tested by our group was liquid spinach. Each team added HCl
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Acids‚ Bases and Buffers Lab Acids‚ Bases and Buffers Lab Results: The experimental results for part one is as follows: Part One Data Table | Initial pH | Final pH | Test Tube A | 6 | 1 | Test Tube B | 4 | 4 | Test Tube C | 4 | ----- | Test Tube D | 4 | 4 | Test Tube E | 6 | 11 | The experimental results for part two is as follows: Part Two Data Table | Before CO2 was Added | After CO2 was Added | Colour | Blue/green | Light green/yellow | pH Level | 8.0pH | 5.0pH |
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Preparation of buffer solutions 1. Activation buffer (Mixed Phosphate Buffer‚ pH 5.5) Solution 1: An accurately weighed quantity of 1.61 g of potassium dihydrogen phosphate was dissolved in sufficient deionized water to produce 100 mL of solution. Solution 2: An accurately weighed quantity of 35.81 g of disodium hydrogen phosphate was dissolved in sufficient volume of deionized water to produce 100 mL. Accurately measured volume of 96.4 mL of solution 1 was mixed with 3.6 mL of solution 2 to get
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Procedure: Day 1: Buffer preparation First‚ the buffer was prepared by using the formula as follows: Figure 1: Calculation for prepare 0.5 M Tris buffer at pH 6.8 3.033 g of Tris was weighed and placed in 400 mL beaker. Then‚ 25 mL of distilled water was added into the beaker that contained Tris. The mixture was dissolved using the stirring rod‚ and then the magnetic stirring bar was placed in the beaker for further dissolve when measuring the pH. The pH meter was used to measure the solution
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I. Introduction A buffer system is a mixture of a weak acid or a weak base and its salt (conjugate base or conjugate acid‚ respectively) that permits solutions to resist large changes in pH upon the addition of small amounts of hydrogen ions (H+) or hydroxide ions (OH-). If the same amount of the buffer is added‚ the pH may only change a fraction of a unit. Our blood is a good example of a buffered system. It is maintained under a pH of 7.4. Thus‚ buffers are important in many areas of chemistry
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Acids‚ Bases‚ and Buffers Introduction: The pH scale is used to determine how acidic or basic a solution is‚ ranging from 1-14. The most acidic of all acids are at a pH level of 1 and the most basic of all bases are at 14. The neutral pH level is 7‚ which is what drinking water is. The pH level is determined by the amount of H+ ions present in a solution‚ and the more H+ ions there are the more acidic it is‚ and the lack of these ions results in more basic solutions. One distinguishing feature
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Buffers CALCULATIONS Table A. pH Measurement using pH meter Calculated pH Solution 1 – HoAc 0.10 M CH3COOH CH3COOH + H2O ⇌ CH3COO- + H3O+ i 0.10 ø ø c -x +x +x e 0.10 – x x x Ka = H3O+[CH3COO-]CH3COOH = x20.10 – x = 1.8 x 10-5 x = 1.33 x 10-3 M pH = -log [1.33 x 10-3] pH = 2.88 Solution 2 – HoAc – OAc
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Performed: June 25‚ 2013 Lab partner: Catalan‚ Christian A. Date Due: July 5‚ 2013 Date Submitted: July 5‚ 2013 EXPERIMENT 1 PREPARING BUFFERS AND BUFFER CAPACITY INTRODUCTION A buffer solution is one in which the pH of the solution is "resistant" to small additions of either a strong acid or strong base. Buffers usually consist of a weak acid and its conjugate base‚ in relatively equal and "large" quantities. A buffer system can be made by mixing
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examine the effectiveness of buffers by titrating two sets of five different solutions using HCl and NaOH and monitoring the pH change of the various solutions. The data collected shows that the buffer systems made with sodium acetate and acetic acid were effect when titrated with the strong acid and the strong base. Comparison of all the solutions shows that the concepts of buffers holds true for the results from the experimentation. Introduction The main objective of this lab was to test the ability
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CHM 116 Lab Investigations of Buffers I. Purpose The purpose of this experiment was to get an understanding as to how to properly prepare chemical buffers. Also part of this experiment was to gauge the effectiveness of the buffers by measuring their pH levels in various titration solutions‚ using a pH meter. II. Procedure To start our experiment we had to prepare Buffer B‚ which was the .060 M Ammonia/Ammonium solution. Using 3.0 M ammonia‚ we had to calculate
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