1) A peptide‚ KKDSRHSTTR is tightly bound to a negatively charged ion exchange column. The column is washed with a pH 7.2 HEPES buffer (50 mM) and the peptide does not elute. Suggest two ways that you can change the buffer that will make the peptide elute from the column. 2) What amino acid(s) is/are involved in crosslinking polypeptide chains. How can these crosslinks be cleaved and prevented from reforming? 3) A polypeptide is subjected to the following degradative techniques results
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RNA Analysis 1. The color result is light brown. 2. A positive Biuret test indicates the presence of proteins in the filtrate. Biuret is used to test for proteins. It is a light blue color and changes to purple in the presence of protein‚ a polymer made of amino acids. It will not change color when only amino acids are present. 3. What type of molecules present in the suspension is hydrolyzed by the mild acid treatment? 4. The products of hydrolysis are peptones‚ peptides‚ and amino acids
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I. Changes Leading to Exploration A. Expulsion of Moors in Spain and reunification of the country under Catholic rulers Isabella and Ferdinand caused an age of exploration and expansion. B. After German Monk Martin Luther posted his 95 theses against church abuses‚ there was a powerful movement away from the Catholic Church called the Protestant Reformation‚ causing the Roman Catholic countries to secure their power in the world by converting
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The reader can gain a deeper understanding of the power struggle between Cameron and his Dad in Going Bovine by Libba Bray by looking through the marxist literary lense. Throughout the book‚ Cameron’s Dad has always been in charge and rules the family through an iron grip. While Cameron’s Dad is cutting the grass‚ Cameron states “Our eyes meet for a nanosecond‚ and then Dad stoops to examine a particularly hearty clump of weeds. As I back the Turdmobile down the driveway‚ he’s running the mower over
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Oct 2012 LSM 1101 Biochemistry of Biomolecules Class 1a: Nucleic acids Our class on DNA is divided into 3 parts: (I) Genetics (II) DNA structure (III) Concepts and applications. I. Genetics: In the primordial period‚ simple molecules were formed from atoms and from these molecules‚ macromolecules were formed. These macromolecules formed life and all living organisms. The classical genetic and heredity observations in the 19th century started the search for the origin of life. The transforming
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Introduction: Dependence of Methane Production on Ambient Temperature in Bovine Waste The purpose of this experiment was to determine the effect of temperature in bovine waste on the amount of methane evolved. The hypothesis stated that the bovine waste heated to 48.9oC would produce the most methane. The manipulated variable in the experiment was the temperature of bovine waste. The responding variable was the amount of methane evolved from the waste. The experiment can be applied to real world
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1. Receptors for touch‚ heat‚ pressure‚ vision‚ and hearing are classified as ___ because they sense stimuli that arise external to the body. 2. Color vision receptors are known as ________ . 3. The posterior pituitary gland secretes __ _ and ___ _. 4. The adult pineal gland is a small‚ shriveled remnant of the child’s. True False 5. In the blood hydrophillic hormones are often bound to transport proteins. True False True False 6. People
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Wernicke Encephalopathy and Korsakoff Syndrome Rasheema Douglas School Of New Resources Wernicke Encephalopathy and Korsakoff Syndrome Wernicke Encephalopathy and Korsakoff Syndrome are the acute and chronic stages of the same disease abbreviated as (WKS). (WKS) is a memory brain disorder that can result if there is a very low amount of Vitamin (B1) or Thiamine entering to the brain
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Biological chemistry 1B Experiment 1: Thin layer chromatography [TLC] Aim The aim of this experiment is to apply the technique of TLC to first separate out a mixture of coloured dyes and then to do the same for selected range of amino acids. Procedure 2 MMs of each different colour were collected into separate beakers. Approximately 5 drops of water is added to each of the beakers containing the MMs and the tablets were stirred until
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Restriction Endonuclease Digestion of DNA from E. coli cells and Analysis by Agarose Gel Electrophoresis Introduction The main goals of this experiment are testing an alternative procedure called “boiling lysis”‚ evaluating the quality of the purified plasmid for restriction digests‚ and identifying the mislabeled plasmid. The plasmid DNA from a carrier E. coli strain was purified by the boiling lysis. In the boiling lysis method‚ the bacterial cells were given momentary heat treatment
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