in Food Lab Stefanie Bogaert Observations Part 1: Test for Proteins Biuret Test for Protein Test Tube Contents Colour Change Conclusions 1 Distilled water Clear to Blue tint There are no proteins present because there was no significant colour change 2 Albumin White translucent to Purple/violet Therefore there are proteins present because of the significant purple colour exposed from the reaction of the Biuret solution 3 Pepsin Brown to Pink/brown There is a little to none trace of proteins
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and the casein and whey proteins exist within milk‚ it can be concluded that whipping cream is composed of proteins as it is made with milk. Similarly‚ it was assumed that soy milk would also contain proteins as it is a vegetable protein. When the Biuret reagent was added to the soy milk‚ the bottom of the test tube became a lilac hue while the top and middle remained as the original cloudy‚ off-white coloured liquid. Soybeans are among the best plant-based proteins‚ as unlike plant proteins‚ soy
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part c is the 5 Ml of distilled water. The purpose of washing the test tubes thoroughly is so you don’t mix substances‚ which can cause your results to be inaccurate. Hypotheses: 1. If the test samples contain Protein‚ then in the presence of Biuret the color will turn to yellow‚ green‚ or lavender. 2. If the test samples contain Carbohydrates‚ then in the presence of Benedict’s color will turn to “traffic light colors” which consist or orange‚ red‚ yellow‚ and sometimes green. 3. If
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tests? The water was tested for each initial chemical test because it served as the controlled variable. Since water is pure oxygen and hydrogen unlike Glucose and Starch‚ one variable may be observed. 2. Biuret reagent will turn your skin brownish-purple. Explain why this occurs. Biuret reagent will turn your skin a brownish-purple because Iodine detects protein in a substance by changing the color to a brown or purple shade‚ and since your skin contains protein it will also change color.
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intact while the other is broken. The different tests that are used in this experiment are : biuret test‚ Sakaguchi test‚ ninhydrin test‚ xanthoproteic test‚ and Hopkins - Cole test. Casein is one of the proteins found in milk belonging to a group called phosphoproteins. It is commonly used as a binding agent in foods. It can easily separated from milk through the introduction of acetic acid. Biuret test is a color reaction test used in determining the presence of peptide bonds in the protein
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We then went to see if the sucrose entered the bag unfortunately the benedict’s solution showed negative results. The second test ( Bag: 5% ovalbumin Beaker: 1 M sucrose) we put biuret in a test tube with the beaker solution and the indicator showed negative results. Then for the next test (Bag: 1 M Glucose Beaker: distilled H2O) they put Benedict’s solution in a test tube with the beaker liquid. The benedicts showed positive results
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Table 1: Biuret results. Substance Tested Predicted Results Biuret Color & Number of drops added 1: Egg white Contain protien 8 drops‚ purple 2: Pepsin none 8 light purple 3: Sugar none 8 none 4: Distilled water none 8 none Questions A. Which of the test tubes are the positive and negative controls? Test tube 1 and 2 are positive controls while 3 and 4 are negative. B. What conclusions can be made about this experiment? Egg white and pepsin contain proteins. C. How might Biuret reagent
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I. Abstract The common organic compounds provide us with nutrients are sugars and starches‚ carbohydrates‚ proteins‚ and lipids. In this lab‚ qualitative tests are performed to identify the presence of organic compounds in food using indicators‚ chemical substances that react in a certain way when a particular substance is present. We put one indicator into the each test tube containing different solutions at a time to see what organic compound is present. The color change in the indicator tells
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Centre Number Candidate Name Candidate Number International General Certificate of Secondary Education CAMBRIDGE INTERNATIONAL EXAMINATIONS BIOLOGY PAPER 6 Alternative to Practical 0610/6 MAY/JUNE SESSION 2002 1 hour Candidates answer on the question paper. No additional materials are required. TIME 1 hour INSTRUCTIONS TO CANDIDATES Write your name‚ Centre number and candidate number in the spaces at the top of this page. Answer all questions. Write your answers in the
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Identifying Organic Compounds 2. Purpose/Problem: I am doing this experiment to find out what kind of organic compounds are in test tubes A‚ B‚ C and D. I am curious to know if substance D was the lipid because it seemed thin and had a slightly yellow tint of color to it. 3. Research: In my research I was informed about facts and appearances of lipids. First of all lipids are non-polar compounds‚ thus they won’t dissolve in water. This is also known as being hydrophobic. They are made with one
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