The Effects of pH‚ Temperature‚ Enzyme‚ and Substrate Concentrations on Benzoquinone Production BIOL 2051 June 10th 2013 Introduction Enzymes are the ultimate catalysts of living things. Enzymes are made of proteins which are structured and directed by amino acids chains. Enzymes attract and fit substrate molecules to an active site. The active site binds the substrate molecules covalently to enzyme forming an enzyme-substrate complex‚ which catalyzes
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Effect of Temperature on Enzymes ~Abstract~ In this experiment the effect of different types of temperatures on enzyme activity was examined. The temperature baths that were used to test the difference in enzyme activity on fresh liver were; 4 °C‚ room temperature which was 22°C‚ body temperature which is 37°C‚ and 77°C. The total time of each trial was 2 and a half minute‚ 1 minute for the H2O2 to acclimatize to the temperature‚ 1 and a half minutes for the reaction to occur. Catalase causes Hydrogen
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INTRODUCTION Enzymes are a protein serving as a catalyst‚ a chemical agent that changes the rate of the reaction without being consumed by the reaction. Enzymes are proteins made up of long chains of amino acids. These form complex shapes. The enzymes are individuals‚ like the different players on a ball team‚ they have different specific structures and jobs. As one ball player may be very tall and one short‚ the specific different shape of the active site on an enzyme is unique and prepares it
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Stallings Period 2 January 26‚ 2013 Enzyme Catalysis Lab Report Background: Enzymes are catalyst‚ which affect the rate of a chemical reaction. One consequence includes the cell to carry out complex chemical activities at relatively low temperatures. In these reactions the substrate binds reversibly to the active site. The cause of this is a decrease in the energy needed to activate the reaction of the substrate molecule to from products. Every enzyme is particular for a reaction for the reason
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Chemistry 512 Enzyme Catalysis Lab Report Pre-lab Questions: 1. Write a balanced chemical equation with state symbols for the reaction catalyzed by peroxidase. 2H2O2 2H2O + O2 (4H1 4O) (4H + 2O + 2O) 2. What is the substrate(s) of this reaction? What is the catalyst? Substrate = H2O2 hydrogen peroxide Catalyst = peroxide 3. At what approximate temperature do enzymes normally operate in the body of a warm-blooded animal? Would your answer change if the enzyme came from a plant or yeast? Enzymes normally
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How Enzymes Work In Different Environments By Sarah Smith Biology1111 October 20‚ 2011 Lab Partner: Nellie Greer ABSTRACT Peroxidase is an enzyme found in potatoes that catalyzes the breakdown of hydrogen peroxide‚ H2O2‚ into O2 gas and water. We examined the different pH environments that can affect the enzyme activity during the breakdown of H2O2. In order to do this‚ we added different levels of pH‚ low‚ medium‚ and high‚ into different test tubes with the enzyme and H2O2‚
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Protocol for Lab 5 – Aerobic Respiration Part 1 Isolation of Mitochondria from Cauliflower - Weigh 50g of rosettes cut from fresh cauliflower head. - Cut rosettes and place it on ice - Prepare juice extractor by placing ice and an empty 150 ml beaker into the right compartment. - Collect pulp from left compartment and record total volume of the extract. Approx. 20ml - Filter the pulp using six layered cheese cloth and collect it in a beaker sitting on ice. - Place two 50 ml test tubes
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LAB 1: What temperature does the enzyme actually work properly in? (Hypothesis) If the temperature is below 40 but above 20‚ then the liver will show bubbles. If the temperature is raised higher than the optimum temperature‚ then an extreme decline in enzyme activity would occur following by the quick denaturing of the enzyme‚ rendering it is permanently useless. Also about 37°C is body temperature. The liver that was at 25°C had a huge amount of bubbles (a 4 on the scale) and the 0°C
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BIO 5 Lab Report: Lactase Enzymes Enzymes are biological catalysts or assistants. Enzymes consist of various types of proteins that work to drive the chemical reaction required for a specific action or nutrient. Enzymes can either launch a reaction or speed it up. The chemicals that are transformed with the help of enzymes are called substrates. In the absence of enzymes‚ these chemicals are called reactants. Enzymes are thought to have an area with a very particular shape. When a molecule of
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Abstract The main goal of the enzyme kinetics experiment was to see how the phosphatase-catalyzed hydrolysis of p-nitrophenyl produced p-nitrophenol in the presence of phosphate and fluoride ion inhibitors of various concentrations. The calculated Km constant was found to be 0.22 for all reactions. The Vmax values for each inhibition ion were 0.00986 for the phosphate ion and 0.00436 for the fluoride ion. The inhibitor constant‚ Ki‚ was determined to be 0.0967 for the phosphate ion. The inhibitor
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