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Work Plan for Isolation of Lactoccocus Lactis

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Work Plan for Isolation of Lactoccocus Lactis
Work Plan for Isolation, Purification, Identification and Starter Culture Activity of Lactoccocus lactis

Submitted by: M.Usman Akram
B.S. (Hons.) Dairy Technology mh.usman@hotmail.com Mobile : +923217773736

University of Veterinary and Animal Sciences,
Ravi Campus Pattoki

Lactoccocus lactis
Classification:
Scientific classification | Kingdom: | Bacteria | Division: | Firmicutes | Class: | Bacilli | Order: | Lactobacillales | Family: | Streptococcaceae | Genus: | Lactococcus | Species: | L. lactis |

Cell Morphology: * L. lactis cells are cocci that group in pairs and form short chains. * Length of L. lactis ranges from 0.5-1.5 micrometer (µm). * L. lactis does not produce spores (nonsporulating). * No capsule formation. * L. lactis grow in microaerophilic conditions * L. lactis are non motile. * L. lactis are Gram (+ive). * L. lactis are catalase (-ive). * L. lactis have low-GC content. * L. lactis are acid-tolerant * Optimum growth temperature is 30oC at pH 6.8. * L. lactis are not produce CO2 in MRS Broth.
Colony Morphology: * No pigment/Color. * Texture is smooth. * No odor. * Pinpoint to small size. * Round form. * Raised elevation. * Smooth margins.
Work Plan for Isolation, Purification, Identification and Starter Culture Activity of Lactoccocus lactis

MATERIAL & METHODS
MATERIAL:
Apparatus: 1. Capped test tubes 2. Test tubes holder stand 3. Beakers 4. Microscope 5. Incubator 6. Platinum loop 7. Microtitre plate 8. Durham tubes 9. Conical Flask 10. Measuring cylinder 11. China dish 12. Funnel 13. Spatula 14. Weighing balance 15. Autoclave 16. Aluminium foil 17. Petri dishes with lids 18. pH meter 19. Burner 20. Pipettes 21. Measuring flask with stopper 22. Colony counter 23. Water bath 24. Anerogen
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