Trypsin Lab Report
INTRODUCTION:
An enzyme is a substance that in most situations, is a catalyst, which increases the rate of reaction by contributing alternative reaction pathways of lower activation energy. Enzymes are able to do this by binding to reactant molecules and allowing them to cooperate in a more energy efficient way. If catalyzed by the appropriate enzyme, reactions that would take an extreme amount of time would rather occur in fractions of a second. First, enzymes increase the rate at which chemical reactions occur without themselves being permanently altered by the reaction. Then, they increase the rate of reaction without adjusting the equilibrium between reactants and products. Throughout this process, water-soluble vitamins act as coenzymes to help catalyze chemical reactions. Hence, enzymes react by compressing the activation energy, thereby …show more content…
It is logical that if the enzyme, Trypsin, functions at the optimum salt concentration of 0.15M, then its ability to function efficiently will decrease. The rationale for this proposed relationship is that Trypsin won’t function in a different concentration than 0.15M because it performs best at that value. Anywhere else it may break down or work at a slower pace.
MATERIALS & METHODS:
To better understand the effect of salt concentration on trypsin the tubes were marked as 2mL with the different salt concentrations. The first for tubes were negative control, the next four tubes were the first trial of trypsin, and the last four tubes were the second trial of trypsin. This step was done to differentiate between the tubes and make sure the solutions are put in the correct place. The micropipette that dials 1000 was set to 045 and 450µL of 0.0M NaCl(pH8) was measured and repeated three times and added to the correct tube. This step was repeated
An enzyme is a substance that in most situations, is a catalyst, which increases the rate of reaction by contributing alternative reaction pathways of lower activation energy. Enzymes are able to do this by binding to reactant molecules and allowing them to cooperate in a more energy efficient way. If catalyzed by the appropriate enzyme, reactions that would take an extreme amount of time would rather occur in fractions of a second. First, enzymes increase the rate at which chemical reactions occur without themselves being permanently altered by the reaction. Then, they increase the rate of reaction without adjusting the equilibrium between reactants and products. Throughout this process, water-soluble vitamins act as coenzymes to help catalyze chemical reactions. Hence, enzymes react by compressing the activation energy, thereby …show more content…
It is logical that if the enzyme, Trypsin, functions at the optimum salt concentration of 0.15M, then its ability to function efficiently will decrease. The rationale for this proposed relationship is that Trypsin won’t function in a different concentration than 0.15M because it performs best at that value. Anywhere else it may break down or work at a slower pace.
MATERIALS & METHODS:
To better understand the effect of salt concentration on trypsin the tubes were marked as 2mL with the different salt concentrations. The first for tubes were negative control, the next four tubes were the first trial of trypsin, and the last four tubes were the second trial of trypsin. This step was done to differentiate between the tubes and make sure the solutions are put in the correct place. The micropipette that dials 1000 was set to 045 and 450µL of 0.0M NaCl(pH8) was measured and repeated three times and added to the correct tube. This step was repeated