Thin Layer Chromatography Lab Report
OBJECTIVE
The purpose of this experiment is to analyze mixtures of compounds prior to, during and after a separation scheme. This experiment also allows monitoring reactions of organic molecules, and determines the identity of a mixture of compounds.
STRUCTURES AND PHYSICAL PROPERTIES OF REACTANTS
[1] SOLVENTS | a. Hexane1Molecular Molarity: 86.18 g/molBoiling Point: 69 ºCMelting Point: -95ºCDensity: 0.659 g/mL at 25ºCWater Solubility: Insoluble in waterColor/Texture: Colorless/LiquidHazardous Information: May cause irritation to the eyes, skin and respiratory system. May cause drowsiness and/or dizziness. Suspected of damaging fertility or unborn child. May cause damage to organs. Toxic to aquatic life. Highly flammable liquid and vapor. May be fatal if swallowed.Structure: CH3(CH2)4CH3 | | b. Methanol2Molecular Molarity: 32.04 g/molBoiling Point: 64.7ºCMelting Point: -98ºCDensity: 0.791 g/mL at 25ºCWater Solubility: Completely miscibleColor/Texture: Colorless/LiquidHazardous Information: Highly flammable liquid and vapor. Toxic if swallowed or in contact with skin. Causes skin and eye irritation. Causes damage to organs.Structure: CH3OH | | c. Methylene Chloride3Molecular Molarity: 84.93 g/molBoiling Point: 39.8-40.0ºCMelting Point: -97ºCDensity: 1.325 g/mL at 25ºCWater Solubility: Slightly soluble in waterColor/Texture: Colorless/LiquidHazardous Information: Harmful if swallowed. Causes skin and eye irritation. Suspected of causing cancer.Structure: CH2Cl2 | 2. Possible Components of Mixture | a. Biphenyl4Molecular Molarity: 154.21 g/molBoiling Point: 255ºCMelting Point: 68-70ºCDensity: 0.992 g/cm3Water Solubility: 0.0075 g/L at 15ºCColor/Texture: Light yellow/CrystallineHazardous Information: May be harmful if swallowed. Causes skin and serious eye irritation. May cause respiratory irritation. Very toxic to aquatic life.Structure: C12H10 | | b. Benzophenone5Molecular Molarity: 182.22 g/molBoiling Point: 305ºCMelting Point: 47-51ºCDensity:
The purpose of this experiment is to analyze mixtures of compounds prior to, during and after a separation scheme. This experiment also allows monitoring reactions of organic molecules, and determines the identity of a mixture of compounds.
STRUCTURES AND PHYSICAL PROPERTIES OF REACTANTS
[1] SOLVENTS | a. Hexane1Molecular Molarity: 86.18 g/molBoiling Point: 69 ºCMelting Point: -95ºCDensity: 0.659 g/mL at 25ºCWater Solubility: Insoluble in waterColor/Texture: Colorless/LiquidHazardous Information: May cause irritation to the eyes, skin and respiratory system. May cause drowsiness and/or dizziness. Suspected of damaging fertility or unborn child. May cause damage to organs. Toxic to aquatic life. Highly flammable liquid and vapor. May be fatal if swallowed.Structure: CH3(CH2)4CH3 | | b. Methanol2Molecular Molarity: 32.04 g/molBoiling Point: 64.7ºCMelting Point: -98ºCDensity: 0.791 g/mL at 25ºCWater Solubility: Completely miscibleColor/Texture: Colorless/LiquidHazardous Information: Highly flammable liquid and vapor. Toxic if swallowed or in contact with skin. Causes skin and eye irritation. Causes damage to organs.Structure: CH3OH | | c. Methylene Chloride3Molecular Molarity: 84.93 g/molBoiling Point: 39.8-40.0ºCMelting Point: -97ºCDensity: 1.325 g/mL at 25ºCWater Solubility: Slightly soluble in waterColor/Texture: Colorless/LiquidHazardous Information: Harmful if swallowed. Causes skin and eye irritation. Suspected of causing cancer.Structure: CH2Cl2 | 2. Possible Components of Mixture | a. Biphenyl4Molecular Molarity: 154.21 g/molBoiling Point: 255ºCMelting Point: 68-70ºCDensity: 0.992 g/cm3Water Solubility: 0.0075 g/L at 15ºCColor/Texture: Light yellow/CrystallineHazardous Information: May be harmful if swallowed. Causes skin and serious eye irritation. May cause respiratory irritation. Very toxic to aquatic life.Structure: C12H10 | | b. Benzophenone5Molecular Molarity: 182.22 g/molBoiling Point: 305ºCMelting Point: 47-51ºCDensity:
References: 1MSDS. Rep. no. 296090. N.p.: n.p., n.d. Sigma Aldrich. Sigma Aldrich. Web. 14 Oct. 2012. <www.sigmaaldrich.com>. 2MSDS. Rep. no. 34860. N.p.: n.p., n.d. Sigma Aldrich. Sigma Aldrich. Web. 14 Oct. 2012. <www.sigmaaldrich.com>. 3MSDS. Rep. no. 270997. N.p.: n.p., n.d. Sigma Aldrich. Sigma Aldrich. Web. 14 Oct. 2012. <www.sigmaaldrich.com>. 4MSDS. Rep. no. W312908. N.p.: n.p., n.d. Sigma Aldrich. Sigma Aldrich. Web. 14 Oct. 2012. <www.sigmaaldrich.com>. 5MSDS. Rep. no. 427551. N.p.: n.p., n.d. Sigma Aldrich. Sigma Aldrich. Web. 14 Oct. 2012. <www.sigmaaldrich.com>. 6MSDS. Rep. no. B4856. N.p.: n.p., n.d. Sigma Aldrich. Sigma Aldrich. Web. 14 Oct. 2012. <www.sigmaaldrich.com>. Benzhydrol can be synthesized from Benzophenone via a reduction reaction. Samples of the reaction mixture are taken at several times during the reaction (start, 50% of the way and at the end of the reaction time). Draw the anticipated results (spots) for silica gel TLC plates at the specified reaction (not elution) times (assume the best solvent used above): At start (no reaction) At 50 % completion At 100 % completion