Summary Of The Sanger Method
Fred Sanger characterized the first insulin protein sequences in 1951. Biologists have since been using these protein sequences to understand how the molecules function. The “Sanger method” was one of the first and best known methods for sequencing DNA and other long strand molecules. The human genome project was later based off the Sanger sequencing. The analysis of sequences of transfer RNAs began in 1969. Correlated changes in the nucleotide sequences were inferred from residue interactions of RNA analysis. The tRNA secondary structure was put forth by these residual interactions. The Needleman–Wunsch algorithm was published in 1970 for dual alignment of sequences. In 1977, Robert Holley from Cornell University along with his team was the