In IC, the analyte is injected into the eluent, which then passes through a column and separates ions between a stationary and mobile phase (Harris & Lucy, chap. 26). Depending on how well the anions stick to the column will determine the retention order of the element. Since the anions that are smaller in size and have higher negative charges will stick to the stationary phase the best, the first peak visible will be Cl-, then NO3-, and lastly SO42-. Unlike the AAS method describe above, the IC tap water sample was not diluted prior to running the sample. Once the sample was analyzed and all three peaks of the elements were found. A one point calibration curve was made from the results of the instructor’s standard by plotting peak area against concentration, which was lastly used to determine the concentration of each
In IC, the analyte is injected into the eluent, which then passes through a column and separates ions between a stationary and mobile phase (Harris & Lucy, chap. 26). Depending on how well the anions stick to the column will determine the retention order of the element. Since the anions that are smaller in size and have higher negative charges will stick to the stationary phase the best, the first peak visible will be Cl-, then NO3-, and lastly SO42-. Unlike the AAS method describe above, the IC tap water sample was not diluted prior to running the sample. Once the sample was analyzed and all three peaks of the elements were found. A one point calibration curve was made from the results of the instructor’s standard by plotting peak area against concentration, which was lastly used to determine the concentration of each