RSM using CCD method
Bioresource Technology 100 (2009) 1192–1196
Contents lists available at ScienceDirect
Bioresource Technology journal homepage: www.elsevier.com/locate/biortech
Optimization of culture conditions for hydrogen production by Ethanoligenens harbinense B49 using response surface methodology
Wan-Qian Guo a, Nan-Qi Ren a,*, Xiang-Jing Wang b, Wen-Sheng Xiang b, Jie Ding a, Yang You a,
Bing-Feng Liu a a b
State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin 150090, PR China
Research Center of Life Science and Biotechnology, Northeast Agricultural University, Harbin 150030, PR China
a r t i c l e
i n f o
Article history:
Received 16 May 2008
Received in revised form 28 July 2008
Accepted 28 July 2008
Available online 14 September 2008
Keywords:
Hydrogen production
Ethanol fermentation
Response surface methodology
Ethanoligenens harbinense B49
Culture conditions
a b s t r a c t
The design of an optimum and cost-efficient medium for high-level production of hydrogen by Ethanoligenens harbinense B49 was attempted by using response surface methodology (RSM). Based on the Plackett–Burman design, Fe2+ and Mg2+ were selected as the most critical nutrient salts. Subsequently, the optimum combination of the selected factors and the sole carbon source glucose were investigated by the Box–Behnken design. Results showed that the maximum hydrogen yield of 2.21 mol/mol glucose was predicted when the concentrations of glucose, Fe2+ and Mg2+ were 14.57 g/L, 177.28 mg/L and
691.98 mg/L, respectively. The results were further verified by triplicate experiments. The batch reactors were operated under an optimized condition of the respective glucose, Fe2+ and Mg2+ concentration of
14.5 g/L, 180 mg/L and 690 mg/L, the initial pH of 6.0 and experimental temperature of 35 ± 1 oC. Without further pH adjustment, the maximum hydrogen yield of 2.20 mol/mol glucose was obtained based on the optimized medium
Contents lists available at ScienceDirect
Bioresource Technology journal homepage: www.elsevier.com/locate/biortech
Optimization of culture conditions for hydrogen production by Ethanoligenens harbinense B49 using response surface methodology
Wan-Qian Guo a, Nan-Qi Ren a,*, Xiang-Jing Wang b, Wen-Sheng Xiang b, Jie Ding a, Yang You a,
Bing-Feng Liu a a b
State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin 150090, PR China
Research Center of Life Science and Biotechnology, Northeast Agricultural University, Harbin 150030, PR China
a r t i c l e
i n f o
Article history:
Received 16 May 2008
Received in revised form 28 July 2008
Accepted 28 July 2008
Available online 14 September 2008
Keywords:
Hydrogen production
Ethanol fermentation
Response surface methodology
Ethanoligenens harbinense B49
Culture conditions
a b s t r a c t
The design of an optimum and cost-efficient medium for high-level production of hydrogen by Ethanoligenens harbinense B49 was attempted by using response surface methodology (RSM). Based on the Plackett–Burman design, Fe2+ and Mg2+ were selected as the most critical nutrient salts. Subsequently, the optimum combination of the selected factors and the sole carbon source glucose were investigated by the Box–Behnken design. Results showed that the maximum hydrogen yield of 2.21 mol/mol glucose was predicted when the concentrations of glucose, Fe2+ and Mg2+ were 14.57 g/L, 177.28 mg/L and
691.98 mg/L, respectively. The results were further verified by triplicate experiments. The batch reactors were operated under an optimized condition of the respective glucose, Fe2+ and Mg2+ concentration of
14.5 g/L, 180 mg/L and 690 mg/L, the initial pH of 6.0 and experimental temperature of 35 ± 1 oC. Without further pH adjustment, the maximum hydrogen yield of 2.20 mol/mol glucose was obtained based on the optimized medium