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Regenerative Biology

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Regenerative Biology
Sophie Harris
Honors Biology
Abstract #4
1/11/2016
Regenerative Biology

Voss J. G., Kump K. D., Walker A. J., Voss S. R. "Variation in Salamander Tail Regeneration Is Associated with Genetic Factors That Determine Tail Morphology." PLOS ONE. Ed. Henry H. Roehl. 3 July 2013.
Available from http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0067274

Scientists Gareth J. Voss, D. Kevin Kump, John A. Walker, S. Randal Voss conducted a study in order to determine tail regenerative growth in salamanders which is passed on by their genes. This explanation of growth would describe the differences in speed, length, width, and shape in different species. These amphibians are known for their skills in regenerating their limbs and parts
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mexicanum/A. andersoni and A. mexicanum (original salamander species). One hundred and twenty days after these hybrids went through fertilization, they went through metamorphosis where they changed from larva to an adult salamander. They were put under anesthesia and had 10% of their tails amputated. After 51 days they had another 1 centimeter of their tails amputated. They were taken care of for 46 more days and then the body weight was recorded and the tails were collected. The tails went through multiple solutions and chemicals to clean and fix them. After this data was recorded, the scientists removed the DNA from the tail tissue and analyzed it. From the data collected, the four scientists were able to determine that male salamanders regenerated much longer tails than females did and also had much larger tail widths than females did. The study also proved that in organisms that took longer to finish going through metamorphosis, the growth and width of the tail were much bigger than in those that had a quicker development or normal development. Additionally, the scientists determined that most differences in growth had to do with sex differences, the speed and timing of development, and the differences in the tail structure. Furthermore, the scientists determined that the speed of the tail regeneration was based on the space between the tip of the tail and the amputation. The closer the amputation, the slower the regeneration process will be.

Location and date of experiment was not available in this article. However, it was published in 2013, so the study was done

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