Rapd Profile, Antioxidant and Antimicrobial Potential in Zingiber Officinale Rosc. Collected from Different Ecological Zones of India
RAPD Profile, Antioxidant and Antimicrobial Potential in Zingiber officinale Rosc. collected from different ecological zones of India
Sapna Sharma1, Jyotsna Singh1 Archana Kumari1, Chetna Mishra1, Poonam Kakkar1*
Herbal Research Section, Indian Institute of Toxicology Research,
P.O. Box No. 80, Lucknow, India
*Correspondence to:
Dr (Mrs) P. Kakkar
Head, Herbal Research
Industrial Toxicology Research Centre
Post Box No.80, M.G. Marg,
Lucknow-226 001, India
Tel: (+91)-0522-2213786, 2627586*269
Fax: (+91)-0522-2628227
E.mail: poonam_kakkar@yahoo.com Abstract
Zingiber officinale Rosc. rhizome, collected from 9 locations of India was explored for any variations in their chemical constituents, antimicrobial and antioxidant potential. RAPD was also performed to observe the relatedness among samples. Maximum camphene, geraniol (3.96, 0.6mg/kg), and total phenolics (65.08 GAE mg/kg) were observed in Madurai sample. Samples had varied antioxidant potential as accessed by SOD mimetic activity, lipid peroxidation inhibition & ABTS•+ scavenging assay. Madurai sample had highest superoxide and ABTS•+ scavenging potential, wheras maximum LPO inhibition was observed in Pune. Extracts showed antimicrobial activities against two gram +ve (B. cereus, S. auerus) aaaaand five gram –ve bacteria (S typhi, S. flexneri, S. sonnei, P. aurginosa and E. coli). Madurai sample had maximum antimicrobial activity against these microorganisms. RAPD profile showed a total 1020 bands scoring 250 RAPD marker bands with 0.31-0.86 similarity. Madurai and Ottapalam samples formed entirely different cluster in dendogram. Variation observed in biological activity may be useful for selecting raw material for therapeutic purposes.
Keywords Ginger (Zingiber officinale Rosc.), Antioxidant activity, Antimicrobial activity, PCR-RAPD, Genetic diversity.
1. Introduction
Ginger (Zingiber officinale) belonging to the family Zingiberaceae is cultivated in various parts of the world,
Sapna Sharma1, Jyotsna Singh1 Archana Kumari1, Chetna Mishra1, Poonam Kakkar1*
Herbal Research Section, Indian Institute of Toxicology Research,
P.O. Box No. 80, Lucknow, India
*Correspondence to:
Dr (Mrs) P. Kakkar
Head, Herbal Research
Industrial Toxicology Research Centre
Post Box No.80, M.G. Marg,
Lucknow-226 001, India
Tel: (+91)-0522-2213786, 2627586*269
Fax: (+91)-0522-2628227
E.mail: poonam_kakkar@yahoo.com Abstract
Zingiber officinale Rosc. rhizome, collected from 9 locations of India was explored for any variations in their chemical constituents, antimicrobial and antioxidant potential. RAPD was also performed to observe the relatedness among samples. Maximum camphene, geraniol (3.96, 0.6mg/kg), and total phenolics (65.08 GAE mg/kg) were observed in Madurai sample. Samples had varied antioxidant potential as accessed by SOD mimetic activity, lipid peroxidation inhibition & ABTS•+ scavenging assay. Madurai sample had highest superoxide and ABTS•+ scavenging potential, wheras maximum LPO inhibition was observed in Pune. Extracts showed antimicrobial activities against two gram +ve (B. cereus, S. auerus) aaaaand five gram –ve bacteria (S typhi, S. flexneri, S. sonnei, P. aurginosa and E. coli). Madurai sample had maximum antimicrobial activity against these microorganisms. RAPD profile showed a total 1020 bands scoring 250 RAPD marker bands with 0.31-0.86 similarity. Madurai and Ottapalam samples formed entirely different cluster in dendogram. Variation observed in biological activity may be useful for selecting raw material for therapeutic purposes.
Keywords Ginger (Zingiber officinale Rosc.), Antioxidant activity, Antimicrobial activity, PCR-RAPD, Genetic diversity.
1. Introduction
Ginger (Zingiber officinale) belonging to the family Zingiberaceae is cultivated in various parts of the world,
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