Pseudomonas Aeruginsa Research Paper
TITLE OF PROJECT: Effects of Chemoattractants of Pseudomonas Aeruginosa Through a Capillary Tube.
PROPOSED COST: $1764.90
ABSTRACT:
This project will show how strong certain bacteria that are commonly found in biofilms attract to Pseudomonas aeruginosa through a capillary tube. It will test ten bacteria to see which bacteria have more chemoattractants with Pseudomonas aeruginosa . These bacteria are all motile, so to come together to form a biofilm, they must lose their ways of mobility somehow. Since a biofilm is just one huge community of bacteria, it would be good to find out if there are ones that are more likely to come to Pseudomonas aeruginosa . It will also be done to see if there will be any difference in the ways …show more content…
The bacteria that would need to be made into the live broth cultures are: GRAM POSITIVE--Mycobacterium tuberculosis, Corynebacterium diphtheriae, Nocardia farcinica, Rhodococcus equi, Streptomyces griseus, GRAM NEGATIVE--Escherichia coli, Salmonella typhi, Vibrio cholorae, Heliobacter pylori, and Shigella dysenteriae . Pseudomonas aeroginosa is needed to be grown on a TSA plate to get be able to get a dry extract from. The first step would be to have the dry extract of pseudomonas at the bottom of the capillary tube and then to stick the capillary tube into a test tube where there is a live broth culture of a certain bacteria at the bottom. This experiment would be repeated two more times for each of the motile …show more content…
To perform the count, determine the magnification needed to recognize the desired cell type. Now systematically count the |
|cells in selected squares so that the total count is 100 cells or so (number of cells needed for a statistically significant count). For |
|large cells this may mean counting the four large corner squares and the middle one. For a dense suspension of small cells you may wish to |
|count the cells in the four 1/25 sq. mm corners plus the middle square in the central square. Always decide on a specific counting patter |
|to avoid bias. For cells that overlap a ruling, count a cell as "in" if it overlaps the top or right ruling, and "out" if it overlaps the |
|bottom or left ruling. |
|A way to determine a particle count using a Neubauer hemocytometer and count 187 particles in the five small squares described. Each square|
|has an area of 1/25 mm-squared (that is, 0.04 mm-squared) and depth of 0.1 mm. The total volume in each square is (0.04)x(0.1) = 0.004 |
|mm-cubed. You have five squares with combined volume of 5x(0.004) = 0.02 mm-cubed. Thus you counted 187 particles in a volume of 0.02
PROPOSED COST: $1764.90
ABSTRACT:
This project will show how strong certain bacteria that are commonly found in biofilms attract to Pseudomonas aeruginosa through a capillary tube. It will test ten bacteria to see which bacteria have more chemoattractants with Pseudomonas aeruginosa . These bacteria are all motile, so to come together to form a biofilm, they must lose their ways of mobility somehow. Since a biofilm is just one huge community of bacteria, it would be good to find out if there are ones that are more likely to come to Pseudomonas aeruginosa . It will also be done to see if there will be any difference in the ways …show more content…
The bacteria that would need to be made into the live broth cultures are: GRAM POSITIVE--Mycobacterium tuberculosis, Corynebacterium diphtheriae, Nocardia farcinica, Rhodococcus equi, Streptomyces griseus, GRAM NEGATIVE--Escherichia coli, Salmonella typhi, Vibrio cholorae, Heliobacter pylori, and Shigella dysenteriae . Pseudomonas aeroginosa is needed to be grown on a TSA plate to get be able to get a dry extract from. The first step would be to have the dry extract of pseudomonas at the bottom of the capillary tube and then to stick the capillary tube into a test tube where there is a live broth culture of a certain bacteria at the bottom. This experiment would be repeated two more times for each of the motile …show more content…
To perform the count, determine the magnification needed to recognize the desired cell type. Now systematically count the |
|cells in selected squares so that the total count is 100 cells or so (number of cells needed for a statistically significant count). For |
|large cells this may mean counting the four large corner squares and the middle one. For a dense suspension of small cells you may wish to |
|count the cells in the four 1/25 sq. mm corners plus the middle square in the central square. Always decide on a specific counting patter |
|to avoid bias. For cells that overlap a ruling, count a cell as "in" if it overlaps the top or right ruling, and "out" if it overlaps the |
|bottom or left ruling. |
|A way to determine a particle count using a Neubauer hemocytometer and count 187 particles in the five small squares described. Each square|
|has an area of 1/25 mm-squared (that is, 0.04 mm-squared) and depth of 0.1 mm. The total volume in each square is (0.04)x(0.1) = 0.004 |
|mm-cubed. You have five squares with combined volume of 5x(0.004) = 0.02 mm-cubed. Thus you counted 187 particles in a volume of 0.02