Protein Analysis
BSC 2010C.LAB TH 7-9:50pm 29 August 2013
Biology Lab Report
Lab #1 –PROTEIN EXTRACTION LAB
I. INTRODUCTION
To begin the process of protein extraction and compare the results in a study, it is necessary to understand the importance of proteins, the process of extraction and how you are using the results to determine a rational conclusion.
First understand proteins and the necessity of studying their impact. Proteins are essential molecules for biological functions and are the stimulant for most of the processes within a living system. They are predominant in the cell (20%) and allow signals to transmit between cells. All proteins share one common feature - a 20 amino acid blueprint, but proteins vary by size and functionality.
Knowing how to correctly and efficiently obtain these proteins and measure the kDa is the second practice. There are a few processes that are necessary to make these experiments feasible. These procedures are detailed below in Section II Lab bullet II STUDIES . Collection of the ideal sample of proteins for subsequent analysis is crucial. Researching the identity of the fish samples then rationalizing the differentials to form a hypothesis is the origin of the conclusion. After researching , a person could hypothesize: Fish that have similar protein profiles share similar characteristics and traits. A conclusion to any hypothesis, after results are collected and graphed, can be derived accurately.
II. LAB
The study of proteins and how they are characterized can be effectively developed by a fairly simple process of extraction and isolation.
I. EXTRACTION & ISOLATION A) Proteins are liberated inside a cell lysate for this type of experiment. After being transferred into microcentrifuge tubes, the slurry will spin in a centrifuge for five minutes separating the mixture to a liquid top and a semi-solid base. Supernatant then is transferred into an individual tube for study.
II.
Biology Lab Report
Lab #1 –PROTEIN EXTRACTION LAB
I. INTRODUCTION
To begin the process of protein extraction and compare the results in a study, it is necessary to understand the importance of proteins, the process of extraction and how you are using the results to determine a rational conclusion.
First understand proteins and the necessity of studying their impact. Proteins are essential molecules for biological functions and are the stimulant for most of the processes within a living system. They are predominant in the cell (20%) and allow signals to transmit between cells. All proteins share one common feature - a 20 amino acid blueprint, but proteins vary by size and functionality.
Knowing how to correctly and efficiently obtain these proteins and measure the kDa is the second practice. There are a few processes that are necessary to make these experiments feasible. These procedures are detailed below in Section II Lab bullet II STUDIES . Collection of the ideal sample of proteins for subsequent analysis is crucial. Researching the identity of the fish samples then rationalizing the differentials to form a hypothesis is the origin of the conclusion. After researching , a person could hypothesize: Fish that have similar protein profiles share similar characteristics and traits. A conclusion to any hypothesis, after results are collected and graphed, can be derived accurately.
II. LAB
The study of proteins and how they are characterized can be effectively developed by a fairly simple process of extraction and isolation.
I. EXTRACTION & ISOLATION A) Proteins are liberated inside a cell lysate for this type of experiment. After being transferred into microcentrifuge tubes, the slurry will spin in a centrifuge for five minutes separating the mixture to a liquid top and a semi-solid base. Supernatant then is transferred into an individual tube for study.
II.
References: "Protein extraction." Bioscience Technology Jan. 2011: 21. Science In Context. Web. 5 Sept. 2013. Benskin, Jon, and Sixue Chen. "Proteomics in the classroom: an investigative study of proteins in microorganisms." The American Biology Teacher 74.4 (2012): 237+. Science In Context. Web. 5 Sept. 2013. Proteins. Films Media Group, 1994. Films On Demand. Web. 05 September 2013. < http://digital.films.com/PortalPlaylists.aspx?aid=6503&xtid=6902 >. http://opsdiagnostics.com/notes/ranpri/Homogenization%20Guide%20ver.1.pdf