Pros And Cons Of Conditional Knockouts
Conditional Knockout Models
Knockout models can be roughly divided into conventional knockout models and conditional knockout models. Although conventional knockouts offer a way to study gene function at a systematic level, many researchers choose a conditional knockout approach instead due to their lethality risk and/or their limitations of analyzing a gene’s role in the organogenesis of a particular tissue. With Cre/loxP technology, which is frequently used to create conditional knockout models, however, these problems can be overcome or circumvented. Below we discuss options for conventional knockout strategies:
Cre/LoxP System-based Targeting
Conditional knockouts can be achieved via a chromosome site-specific recombinant enzyme system,
Knockout models can be roughly divided into conventional knockout models and conditional knockout models. Although conventional knockouts offer a way to study gene function at a systematic level, many researchers choose a conditional knockout approach instead due to their lethality risk and/or their limitations of analyzing a gene’s role in the organogenesis of a particular tissue. With Cre/loxP technology, which is frequently used to create conditional knockout models, however, these problems can be overcome or circumvented. Below we discuss options for conventional knockout strategies:
Cre/LoxP System-based Targeting
Conditional knockouts can be achieved via a chromosome site-specific recombinant enzyme system,