Physicochemical Properties Of Bpm I-1-7 Case Study
3. Result and discussion
2.1 Physicochemical properties The formations of BPM I-III were confirmed by FT-IR, powder XRD and SEM studies.
2.1.1 Fourier transform infrared spectroscopy
FT-IR spectra of BPM I-BPM III (Fig. 1a-1c) exhibited characteristic absorption bands of CTS ranges from 1637-1639 cm−1 which are attributed to amide I bands (C=O stretching mode along with an N–H deformation mode). Broad bands from 1130-978 cm−1, 1131-975 cm−1 and 1131-971 cm−1 were observed for phosphate stretching vibration in nano-HAP. The bands at 3438, 3435 and 3436 cm−1 were assigned to the hydroxyl groups of CTS in BPM I, BPM II, BPM III respectively. The presence of PEG in BPM I-III was confirmed from the C-H aliphatic stretching frequency at 2937 cm−1, …show more content…
The temperature dependence of the magnetization of BPM I was shown in Fig. 7. The data was taken in a zero-field-cooling–field-heating sequence. The blocking temperature (TB) observed from this measurements is 212 K. Interestingly, another lower temperature transition at 6.05 K was also observed. At first, magnetization decreased as the temperature rises from 2 K, reached a minimum near critical phase transition temperature (TC) of 6.05 K, and then started increasing from this point to the blocking temperature TB at 212 K.
Fig. 8 showed field-dependent magnetization measurement of BPM I where magnetization (emu/g) as a function of applied field (Oe) was depicted in with the confined field from -10000 to 10000 Oe. The curves showed low coercive field of 22 Oe at body temperature, typical of superparamagnetic behavior, indicated that the presence of nano-Fe3O4 in HAP led to the superparamagnetism of the scaffolds (Wu, Jiang, & Wen, 2010). Diamagnetic background was observed which originates from the CTS-PEG and nano-HAP-Fe3O4. Spontaneous remanent magnetization was observed at 1 x 10-2 …show more content…
FT-IR studies and X-ray diffraction study confirmed the presence of individual components. SEM revealed highly interconnected macro and micro-porous structure which is likely to favor cell adhesion and attachment with nutrient delivery to tissue regeneration site. The porous magnetic nanocomposites exhibited excellent mechanical properties (e.g. tensile strength, Young’s modulus and stiffness). The water uptake ability of the nanocomposites was found to increase with increasing the proportion of PEG. These nanocomposites also showed good antimicrobial effect. Notably, porosities and tensile strengths of the nanocomposite were in the range of cancellous bone. Moreover, superparamagnetic nature of these nanocomposites was observed, which make these materials suitable for magnetic therapy. Finally, these nanocomposites supported for human osteoblast-like MG-63 cells growth, attachment and proliferation without having any negative effect on MG-63 cells and showed good cytocompatibility, suggesting a positive prospect for bone regeneration or repair of bone defects. All the above results suggest that these nanocomposites have great potential to be used as bone tissue engineering
2.1 Physicochemical properties The formations of BPM I-III were confirmed by FT-IR, powder XRD and SEM studies.
2.1.1 Fourier transform infrared spectroscopy
FT-IR spectra of BPM I-BPM III (Fig. 1a-1c) exhibited characteristic absorption bands of CTS ranges from 1637-1639 cm−1 which are attributed to amide I bands (C=O stretching mode along with an N–H deformation mode). Broad bands from 1130-978 cm−1, 1131-975 cm−1 and 1131-971 cm−1 were observed for phosphate stretching vibration in nano-HAP. The bands at 3438, 3435 and 3436 cm−1 were assigned to the hydroxyl groups of CTS in BPM I, BPM II, BPM III respectively. The presence of PEG in BPM I-III was confirmed from the C-H aliphatic stretching frequency at 2937 cm−1, …show more content…
The temperature dependence of the magnetization of BPM I was shown in Fig. 7. The data was taken in a zero-field-cooling–field-heating sequence. The blocking temperature (TB) observed from this measurements is 212 K. Interestingly, another lower temperature transition at 6.05 K was also observed. At first, magnetization decreased as the temperature rises from 2 K, reached a minimum near critical phase transition temperature (TC) of 6.05 K, and then started increasing from this point to the blocking temperature TB at 212 K.
Fig. 8 showed field-dependent magnetization measurement of BPM I where magnetization (emu/g) as a function of applied field (Oe) was depicted in with the confined field from -10000 to 10000 Oe. The curves showed low coercive field of 22 Oe at body temperature, typical of superparamagnetic behavior, indicated that the presence of nano-Fe3O4 in HAP led to the superparamagnetism of the scaffolds (Wu, Jiang, & Wen, 2010). Diamagnetic background was observed which originates from the CTS-PEG and nano-HAP-Fe3O4. Spontaneous remanent magnetization was observed at 1 x 10-2 …show more content…
FT-IR studies and X-ray diffraction study confirmed the presence of individual components. SEM revealed highly interconnected macro and micro-porous structure which is likely to favor cell adhesion and attachment with nutrient delivery to tissue regeneration site. The porous magnetic nanocomposites exhibited excellent mechanical properties (e.g. tensile strength, Young’s modulus and stiffness). The water uptake ability of the nanocomposites was found to increase with increasing the proportion of PEG. These nanocomposites also showed good antimicrobial effect. Notably, porosities and tensile strengths of the nanocomposite were in the range of cancellous bone. Moreover, superparamagnetic nature of these nanocomposites was observed, which make these materials suitable for magnetic therapy. Finally, these nanocomposites supported for human osteoblast-like MG-63 cells growth, attachment and proliferation without having any negative effect on MG-63 cells and showed good cytocompatibility, suggesting a positive prospect for bone regeneration or repair of bone defects. All the above results suggest that these nanocomposites have great potential to be used as bone tissue engineering