Okays
ENTERICS
EMB, SSA, BSA
[pic]
Acronym: T LMI CUP MNL GARI
( TSI, LIA, MIO, Indole, Citrate, Urease, PAD, Malonate, Lactose, Glucose, Arabinose, Rhamnose, Inosite)
easy steps to identify:
*TSI: A/Ag------ Escherichia coli and Klebsiella pneumoniae
A/Ag( yellow both slant and butt with gas: cracks/displacement of the agar) EMB- greenish metallic sheen ------ E. coli
EMB- pink mucoid colonies -------- K. pneumoniae
e.coli (left) k.pneumoniae (right)
*TSI ALK/Ag-----Serratia marcecens, Salmonella paratyphi A
red slant, yellow butt with cracks or agar displacement or bubbles
if with:
King's A with red pigment (prodigiosin: non water soluble)
and LIA is P/P ( purple slant and butt) ------ S. marcecens
-------------->
if LIA is is P/Y (purple slant with yellow butt) --------- S. paratyphi A
*TSI: ALK/AG w/ H2S ---- Proteus vulgaris, Salmonella typhimurium
-->TSI butt and slant all black in color (rightmost tube)
[pic]
if LIA is R/Y (red slant, yellow butt) ----- P.vulgaris and PAD+
if LIA is P/P (purple slant and butt) ------- S. typhimuriuM
*TSI: ALK/A ------- Shigella sonnei, Shigella dysenteriae
[pic]
(red slant, yellow butt without cracks or displacement or bubbles)
both are nonmotile
if MIO the whole agar is purple (OD+) ------ S. sonnei
if MIO's upper part is a little bit purplish or yellow (OD-)---S.dysenteriae
rightmost tube: nonmotile, OD-
nonmotile, OD-
*TSI: ALK/A with H2S ------- Salmonella typhi
[pic]
only enteric sa first 10 na TSI: red slant with blackening, yellow butt
BSA: JET BLACK COLONIES
[pic]
*TSI: ALK/ALK------ Psuedomonas
EMB, SSA, BSA
[pic]
Acronym: T LMI CUP MNL GARI
( TSI, LIA, MIO, Indole, Citrate, Urease, PAD, Malonate, Lactose, Glucose, Arabinose, Rhamnose, Inosite)
easy steps to identify:
*TSI: A/Ag------ Escherichia coli and Klebsiella pneumoniae
A/Ag( yellow both slant and butt with gas: cracks/displacement of the agar) EMB- greenish metallic sheen ------ E. coli
EMB- pink mucoid colonies -------- K. pneumoniae
e.coli (left) k.pneumoniae (right)
*TSI ALK/Ag-----Serratia marcecens, Salmonella paratyphi A
red slant, yellow butt with cracks or agar displacement or bubbles
if with:
King's A with red pigment (prodigiosin: non water soluble)
and LIA is P/P ( purple slant and butt) ------ S. marcecens
-------------->
if LIA is is P/Y (purple slant with yellow butt) --------- S. paratyphi A
*TSI: ALK/AG w/ H2S ---- Proteus vulgaris, Salmonella typhimurium
-->TSI butt and slant all black in color (rightmost tube)
[pic]
if LIA is R/Y (red slant, yellow butt) ----- P.vulgaris and PAD+
if LIA is P/P (purple slant and butt) ------- S. typhimuriuM
*TSI: ALK/A ------- Shigella sonnei, Shigella dysenteriae
[pic]
(red slant, yellow butt without cracks or displacement or bubbles)
both are nonmotile
if MIO the whole agar is purple (OD+) ------ S. sonnei
if MIO's upper part is a little bit purplish or yellow (OD-)---S.dysenteriae
rightmost tube: nonmotile, OD-
nonmotile, OD-
*TSI: ALK/A with H2S ------- Salmonella typhi
[pic]
only enteric sa first 10 na TSI: red slant with blackening, yellow butt
BSA: JET BLACK COLONIES
[pic]
*TSI: ALK/ALK------ Psuedomonas