Nt1330 Unit 2 Lab Summary
Lab Practicum, Micro 200, study guide
When you did your hand wash experiment, you decided a variable to test, say, hot vs cold water, you called this variable an “experimental variable” since it was the variable you were testing experimentally. In order to get meaningful data, you need to keep other things the same, for example the washing time had to be the same for both, you called the washing time a _Control_______ variable.
Through the semester, you have used two bacteria that produce colorful colonies: one produce yellow colonies, another one pink. What are the names of these two bacteria? Are they Gram+ or Gram-?
Micrococcus Luteus, yellow colonies, gram +
Serratia Marcescens, pink colonies, gram -
There was one bacterial …show more content…
Which to observe the cell membrane/nuclear membrane of a cell (microbe)?
Dark field: Motility
Electron Microscope: cell membrane and nuclear membrane.
On each lens of your microscope, there is a # showing the magnification power (4X, 10X, 40X, 100X), there is another # (_____numerical_____ aperture) you use to calculate the resolution power of that lens, do you know the difference between “magnification” and “resolution” power?
Magnification: Makes image bigger to see detail.
Resolution: Clarity of an object, resolution is more important than magnification.
You have learnt many staining techniques through the lab exercises:
What dye did you use in simple stain? Crystal Violet
What dyes did you used in Gram stain? Crystal violet, gram’s iodine, and safranin violet.
Why did you have to heat up the slide when you did the acid fast stain and the spore stain?
To open the spores and allow the dye to enter and stain. Once cooled down the spores closed trapping the dye inside.
What color is a “+” in acid fast stain? Which bacteria are “+” for acid fast stain? What color is a “+” in spore stain? Which bacteria are “+” for acid fast
When you did your hand wash experiment, you decided a variable to test, say, hot vs cold water, you called this variable an “experimental variable” since it was the variable you were testing experimentally. In order to get meaningful data, you need to keep other things the same, for example the washing time had to be the same for both, you called the washing time a _Control_______ variable.
Through the semester, you have used two bacteria that produce colorful colonies: one produce yellow colonies, another one pink. What are the names of these two bacteria? Are they Gram+ or Gram-?
Micrococcus Luteus, yellow colonies, gram +
Serratia Marcescens, pink colonies, gram -
There was one bacterial …show more content…
Which to observe the cell membrane/nuclear membrane of a cell (microbe)?
Dark field: Motility
Electron Microscope: cell membrane and nuclear membrane.
On each lens of your microscope, there is a # showing the magnification power (4X, 10X, 40X, 100X), there is another # (_____numerical_____ aperture) you use to calculate the resolution power of that lens, do you know the difference between “magnification” and “resolution” power?
Magnification: Makes image bigger to see detail.
Resolution: Clarity of an object, resolution is more important than magnification.
You have learnt many staining techniques through the lab exercises:
What dye did you use in simple stain? Crystal Violet
What dyes did you used in Gram stain? Crystal violet, gram’s iodine, and safranin violet.
Why did you have to heat up the slide when you did the acid fast stain and the spore stain?
To open the spores and allow the dye to enter and stain. Once cooled down the spores closed trapping the dye inside.
What color is a “+” in acid fast stain? Which bacteria are “+” for acid fast stain? What color is a “+” in spore stain? Which bacteria are “+” for acid fast