Nt1310 Lab 1
KEY: ABE Lab 1:
Prelab:
Page A17, Questions 1 and 2:
1. What does the term genetic disease mean? What examples of generic diseases do you know about?
A genetic disease is a disease caused by an absent of defective gene in the DNA. Students may know of sickle cell anemia, which is a disease resulting from defective red blood cells; treatment of sickle cell anemia focuses primarily on relieving pain and controlling infections and other complications that may occur.
2. Adding human DNA to bacteria makes it possible to make human insulin. What do you already know about DNA? Be as detailed as possible and discuss the location of DNA in the cell, DNA structure, the replication of DNA, and the components of DNA.
Location of DNA in the cell: in eukaryotic cells, DNA is found in chromosomes in the nucleus and in mitochondria and chloroplasts. In prokaryotic cells, …show more content…
3. Study your gel electrophoresis results:
a) Which solution sample contained a single dye: S1, S2, or S3? How do you know? S3 contained a single dye because it only had one band.
b) The molecular weights for the dyes are 452.38 atomic units (au) for orange G, 669.98 au for bromophenol blue, and 538.62 au for xylene cyanole. How do these weights compare with your original conclusions about the weights of the dyes?
Base on the order of movement of the dyes through the gel (yellow moved the farthest, then purple, then blue), students should have originally predicted that the orange G (yellow dye) was the smallest, then bromophenol blue (purple), then xylene cyanole (blue) was the largest. But based on the molecular weights above, the purple was actually bigger than the blue, yet it moved farther in the gel. View the image below.
4) Do you think there was a difference in the amount of charge on one of the dye molecules? Explain the reasoning for your
Prelab:
Page A17, Questions 1 and 2:
1. What does the term genetic disease mean? What examples of generic diseases do you know about?
A genetic disease is a disease caused by an absent of defective gene in the DNA. Students may know of sickle cell anemia, which is a disease resulting from defective red blood cells; treatment of sickle cell anemia focuses primarily on relieving pain and controlling infections and other complications that may occur.
2. Adding human DNA to bacteria makes it possible to make human insulin. What do you already know about DNA? Be as detailed as possible and discuss the location of DNA in the cell, DNA structure, the replication of DNA, and the components of DNA.
Location of DNA in the cell: in eukaryotic cells, DNA is found in chromosomes in the nucleus and in mitochondria and chloroplasts. In prokaryotic cells, …show more content…
3. Study your gel electrophoresis results:
a) Which solution sample contained a single dye: S1, S2, or S3? How do you know? S3 contained a single dye because it only had one band.
b) The molecular weights for the dyes are 452.38 atomic units (au) for orange G, 669.98 au for bromophenol blue, and 538.62 au for xylene cyanole. How do these weights compare with your original conclusions about the weights of the dyes?
Base on the order of movement of the dyes through the gel (yellow moved the farthest, then purple, then blue), students should have originally predicted that the orange G (yellow dye) was the smallest, then bromophenol blue (purple), then xylene cyanole (blue) was the largest. But based on the molecular weights above, the purple was actually bigger than the blue, yet it moved farther in the gel. View the image below.
4) Do you think there was a difference in the amount of charge on one of the dye molecules? Explain the reasoning for your