Objectives: There were two major goals of the “Observing Bacteria and Blood” experiment. The first was to establish a familiarity with the proper techniques for using a microscope. The experiment focused on both the basic components of a microscope (i.e. how to adjust the knobs and levers for the desired result) as well as how do decide the proper objective to use for observing a specimen. The second goal major goal of this experiment was to practice observing live specimens (yogurt and blood).
Procedures:
Exercise 1: Observation of prepared slides using the microscope. The observation of the 8 prepared slides was performed by making adjustments to the microscope (focus, condenser, light) in order to establish a clear image …show more content…
● Contrast: The darkness of the back ground relative to the specimen.
B. What is the purpose of immersion oil? To direct the light from the microscope directly to the slide and stop it from refracting. It creats a finer resolution and brightness.
Exercise 2: Observing Bacteria Cultures in Yogurt Questions
A. Describe your observations of the fresh yogurt slide.
B. Were there observable differences between your fresh yogurt slide and the prepared yogurt slide? If so, explain.
C. Describe the four main bacterial shapes.
Cocci – oval or spherical shaped. Bacillus – are rod shaped. Spirillum – are thick, rigid spirals. Vibrio – are curved or a comma shaped rod.
D. What are the common arrangements of bacteria? Diplo – oval shaped, found in pairs.
Strepto – are cocci that arange into chains. Staphylo – are cocci that are arranged into irregular clusters, similar to grapes.
E. Were you able to identify specific bacterial morphologies on either yogurt slide? If so, which types?
Exercise 3: Preparing andObservingaBloodSlide