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BASIC MICROBIOLOGY

SMS

ASSIGNMENT 1
(LACTOPHENOL BLUE STAINING)

NAME : 1) FARAH ANINI BINTI MD. SHAFI’I 2) FATIN NUR SYAHIRAH BINTI MOHD. HANAFI 3) NOR AZAH BINTI MAHIZAN 4) JOSHUA RAJ A/L ISIDORE SAKAYARAJ 5) CHAN POH SHIN

MATRIX NO : 1) 012012051710 2) 012012051610 3) 012012051646 4) 012012051472 5) 012012111185

SEMESTER : 2
PROGRAMME : BACHELOR OF NUTRITION (BNT)
LECTURER : MADAM SASIMALANI SARGUNNAM

INTRODUCTION
Figure 1: Common fungi
The Kingdom Fungi includes some of the most important organisms, both in terms of their ecological and economic roles. By breaking down dead organic material, they continue the cycle of nutrients through ecosystems. In addition, most vascular plants could not grow without the symbiotic fungi, or mycorrhizae, that inhabit their roots and supply essential nutrients. Other fungi provide numerous drugs such as penicillin and other antibiotics, foods like mushrooms, truffles and morels, and the bubbles in bread, champagne, and beer.
Fungi also cause a number of plant and animal diseases: in humans, ringworm, athlete's foot, and several more serious diseases are caused by fungi. Fungi are more chemically and genetically similar to animals than other organisms, thus this makes fungal diseases very difficult to treat. Plant diseases caused by fungi include rusts, smuts, and leaf, root, and stem rots, and may cause severe damage to crops. However, a number of fungi, in particular the yeasts, are important "model organisms" for studying problems in genetics and molecular biology.
The lactophenol blue staining or known as lacto phenol cotton blue (LPCB) wet mount preparation is the most widely used method of staining and observing fungi and is simple to prepare. The preparation has three components: phenol, which will kill any live organisms. The high concentration of the phenol deactivates lytic cellular enzymes thus the cells do not lyse.

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