Microscope Lab
Virtual Lab Unit 3
1. Briefly describe the steps required to aseptically transfer bacteria from an unknown to a tube of liquid broth. You might have to research this using the "T" button (Tell Me More About...)
To aseptically transfer bacteria from an unknown to a tube of liquid broth you have to flame the loop or wire before you begin to sterilize it. You then remove the caps from the tubes and flame the mouths of the tubes to prevent air-borne contamination. When all this is done, you have to pick up the inoculum or unknown culture by running the sterile loop or wire down into the tube. Once you got the inoculum you drop it into the sterile medium to inoculate it.
2. What is the purpose of aseptic technique?
The purpose of using the aseptic technique is to prevent contamination when handling your cultures.
3. Does your lab report contain any messages when your inoculation was not complete? What change in the traffic signals indicates an unsuccessful inoculation?
The lab report does not mention anything about inoculation. There’s only a reference if auto-inoculation was used. If inoculation was unsuccessful, the light turns red
4. Does you lab report contain any messages when you don't follow aseptic procedures for transferring bacteria? What change in the traffic signal indicates contamination?
The lab report does not contain any message when aseptic procedures were not followed. The traffic signals turn red when contamination occurs.
5. What indication is there that the loop has been sterilized?
When it is flamed for a few seconds and is sterile, the loop turns red.
6. How much time is provided for users of VUMIE 2012 before the Contamination light turns red? You will have to just time this and be specific, i.e. how many seconds, minutes, etc.
58 seconds is provided for users before the contamination light turns red.
7. In regards to Phenol Red Sorbitol:
What was the name of the test that used Phenol Red Sorbitol?
Sorbitol Fermentation
1. Briefly describe the steps required to aseptically transfer bacteria from an unknown to a tube of liquid broth. You might have to research this using the "T" button (Tell Me More About...)
To aseptically transfer bacteria from an unknown to a tube of liquid broth you have to flame the loop or wire before you begin to sterilize it. You then remove the caps from the tubes and flame the mouths of the tubes to prevent air-borne contamination. When all this is done, you have to pick up the inoculum or unknown culture by running the sterile loop or wire down into the tube. Once you got the inoculum you drop it into the sterile medium to inoculate it.
2. What is the purpose of aseptic technique?
The purpose of using the aseptic technique is to prevent contamination when handling your cultures.
3. Does your lab report contain any messages when your inoculation was not complete? What change in the traffic signals indicates an unsuccessful inoculation?
The lab report does not mention anything about inoculation. There’s only a reference if auto-inoculation was used. If inoculation was unsuccessful, the light turns red
4. Does you lab report contain any messages when you don't follow aseptic procedures for transferring bacteria? What change in the traffic signal indicates contamination?
The lab report does not contain any message when aseptic procedures were not followed. The traffic signals turn red when contamination occurs.
5. What indication is there that the loop has been sterilized?
When it is flamed for a few seconds and is sterile, the loop turns red.
6. How much time is provided for users of VUMIE 2012 before the Contamination light turns red? You will have to just time this and be specific, i.e. how many seconds, minutes, etc.
58 seconds is provided for users before the contamination light turns red.
7. In regards to Phenol Red Sorbitol:
What was the name of the test that used Phenol Red Sorbitol?
Sorbitol Fermentation