Microbiology Lab Assignment
Shakira Jarvis
Microbiology Lab Assignment
Laboratory Assignment Outline
1. Check in & The Microscope a. Review of proper lab etiquette. i. Review laboratory syllabus and b. Review of the Parts of a Microscope ii. Review of lab exercises about different types of Microscopes 2. Survey of Microorganisms c. Viewing, drawing, and describing several types of fungi, algae, and Bacteria iii. Chlamydomonas iv. Spirogyra v. Penicillum mycelium condidiophores vi. Cladophora vii. Volvox viii. Paramecium caudatum ix. Escherichia coli x. Bacillus subtilis xi. Staphylococcus aureus xii. Aspergillus condidiophores xiii. Amoeba Proteus …show more content…
Tests done Test | Purpose | Reagents | Result (Positive) | Result (Negative) | Gram Stain | 1. To determine the cell wall formation ( thick layer peptidoglycan or thin layer peptidoglycan) | Crystal violet, iodine, 95% Alcohol, Safranin | Purple specimen- Gram Positive, cell wall has thick peptidoglycan layer | Pink Specimen- Gram Negative, cell wall has thin peptidoglycan layer | Catalase Test | To determine the production of catalase enzyme. The enzyme that catalyzes H2O2 | Hydrogen Peroxide | Bubbles produced – enzyme present | No bubbles – enzyme not present | Oxidase Test | To determine the presence of cytochrome oxidase. Enzyme used when O2 is used as a terminal electron acceptor | Tetramethyl- pphenylenediamine | Color change from yellow to purple – cytochrome present | No change, reagent remains yellow- cytochrome not present | Glucose Sugar Fermentation ( w/ Durham tube) | To determine if bacteria can ferment glucose | | Color change from yellow to red – acid produced AND/OR Media displacement (bubble) in Durham tube. – gas produced | No color change – no acid productionAND OR No media displacement – no gas production
Microbiology Lab Assignment
Laboratory Assignment Outline
1. Check in & The Microscope a. Review of proper lab etiquette. i. Review laboratory syllabus and b. Review of the Parts of a Microscope ii. Review of lab exercises about different types of Microscopes 2. Survey of Microorganisms c. Viewing, drawing, and describing several types of fungi, algae, and Bacteria iii. Chlamydomonas iv. Spirogyra v. Penicillum mycelium condidiophores vi. Cladophora vii. Volvox viii. Paramecium caudatum ix. Escherichia coli x. Bacillus subtilis xi. Staphylococcus aureus xii. Aspergillus condidiophores xiii. Amoeba Proteus …show more content…
Tests done Test | Purpose | Reagents | Result (Positive) | Result (Negative) | Gram Stain | 1. To determine the cell wall formation ( thick layer peptidoglycan or thin layer peptidoglycan) | Crystal violet, iodine, 95% Alcohol, Safranin | Purple specimen- Gram Positive, cell wall has thick peptidoglycan layer | Pink Specimen- Gram Negative, cell wall has thin peptidoglycan layer | Catalase Test | To determine the production of catalase enzyme. The enzyme that catalyzes H2O2 | Hydrogen Peroxide | Bubbles produced – enzyme present | No bubbles – enzyme not present | Oxidase Test | To determine the presence of cytochrome oxidase. Enzyme used when O2 is used as a terminal electron acceptor | Tetramethyl- pphenylenediamine | Color change from yellow to purple – cytochrome present | No change, reagent remains yellow- cytochrome not present | Glucose Sugar Fermentation ( w/ Durham tube) | To determine if bacteria can ferment glucose | | Color change from yellow to red – acid produced AND/OR Media displacement (bubble) in Durham tube. – gas produced | No color change – no acid productionAND OR No media displacement – no gas production