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Locomotor Activity Lab Report

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Locomotor Activity Lab Report
Locomotor activity The Locomotor activity (Pákáski and Kálmán, 2008) refers to the movement of spontaneous locomotor and exploratory activity in rodents. LMA is a simple performed measurement of behavior to detect the effects of drugs, genetic manipulation, etc., on motor function by measuring horizontal, vertical and stereotyped behavior in rodents. Several measures of locomotor activity in rats, including the distance covered, movement time and speed of progression. This test is sensitive to hippocampal and basal ganglia damage, as well as to hind limb dysfunction (Brudzynski and Krol, 1997). This test will be assessed in an illuminated square arena box (60x60x30 cm.) surrounded by walls to prevent escape. Speed and time spent moving …show more content…
The pool will be located in a procedure room. The circular pool (140cm diameter and 80cm height) contained with water. A platform (20 cm diameter and 40cm height) will be set inside the pool, which will be filled with water to a height 42cm at 22 ± 2 °C and conceptually divided into four quadrants and four points designed as starting positions (N, S, E, or W). The platform will be located in a constant position in the middle of one quadrant equidistant from the center and the edge of the pool and it will be submerged 2cm below the water surface. The water will be added with cassava powder. In each trial, the rat will be placed in the water facing the wall of the pool, in one of the four possible starting locations. The position of starting points will be locked and the series will be repeated in each day. Animals will be received 1 trial/day. This procedure will be repeated for 6 training days. The animal will be allowed to search for the platform for 90 s, and gently guided to it, if it cannot reach the target. If the rat found the platform, it will be allowed to remain there for 15 s and then returned to its home cage. If the rat will be unable to find the platform within 90 s the training session will be finished and the maximum score of 90 s will be
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