Liver And Hydrogen Peroxide Lab Report
Biology investigation: Liver and hydrogen peroxide
By Cuong Tran
Abstract: The purpose of this investigation was to determine the optimum temperature for enzyme activity. The stated hypothesis was that 37oC would be the optimum temperature for liver enzymes to break down hydrogen peroxide into water and oxygen. The dependent variable was to measure the amount of foam being produced, while testing 4 different temperatures with 3 repetitions each. Out of the 12 trials only 2 outliers were found and an inclusive overlook of random and systematic errors were explode to determine the possible causes. The final results support the hypothesis that 37oC was indeed the optimum temperature for enzyme activity, producing the greatest volume of foam …show more content…
The bar graph above represents a visual overall look of how temperature affects the reaction rate. The dependent variable was to measure the volume of foam been produced at each temperature with 3 repetitions. The graph shows that 37oC was the optimum temperature and producing the greatest height of reaction 44mL. However, as the temperature continues to increase the enzymes begin to denature resulting in the lowest recorded volume of 15mL at 70oC. Although the results do support the hypothesis for 37oC the standard deviation was 6.29, while 50oC had a standard deviation of 4.15. Out of the 12 trials 2 outliers were apparent 2nd trial at 37oC and 1st trial at 50oC which may have been caused by random and systematic errors throughout the …show more content…
This investigation could have been improved if more trials were tested, around 4 to 6, this would ensure a better overall average of each temperature and standard deviation to the true value. Adding in 1 or 2 more set temperatures to be tested such as 20oC and 40oC would also give an overall overlook of the reaction rate of enzymes. Where reaction of enzyme activity is at its peak and then when it begins to denature. Another improvement would be to concentrate on one water bath at a time. While testing the reaction rate in one water bath the other may have when left unattended either cooled off or warmed up. Because some of the data was varied compared to other groups, there may of have been an error with the liver itself. While the pure samples of liver were the same surface area, not all pieces were blended from the same section of the
By Cuong Tran
Abstract: The purpose of this investigation was to determine the optimum temperature for enzyme activity. The stated hypothesis was that 37oC would be the optimum temperature for liver enzymes to break down hydrogen peroxide into water and oxygen. The dependent variable was to measure the amount of foam being produced, while testing 4 different temperatures with 3 repetitions each. Out of the 12 trials only 2 outliers were found and an inclusive overlook of random and systematic errors were explode to determine the possible causes. The final results support the hypothesis that 37oC was indeed the optimum temperature for enzyme activity, producing the greatest volume of foam …show more content…
The bar graph above represents a visual overall look of how temperature affects the reaction rate. The dependent variable was to measure the volume of foam been produced at each temperature with 3 repetitions. The graph shows that 37oC was the optimum temperature and producing the greatest height of reaction 44mL. However, as the temperature continues to increase the enzymes begin to denature resulting in the lowest recorded volume of 15mL at 70oC. Although the results do support the hypothesis for 37oC the standard deviation was 6.29, while 50oC had a standard deviation of 4.15. Out of the 12 trials 2 outliers were apparent 2nd trial at 37oC and 1st trial at 50oC which may have been caused by random and systematic errors throughout the …show more content…
This investigation could have been improved if more trials were tested, around 4 to 6, this would ensure a better overall average of each temperature and standard deviation to the true value. Adding in 1 or 2 more set temperatures to be tested such as 20oC and 40oC would also give an overall overlook of the reaction rate of enzymes. Where reaction of enzyme activity is at its peak and then when it begins to denature. Another improvement would be to concentrate on one water bath at a time. While testing the reaction rate in one water bath the other may have when left unattended either cooled off or warmed up. Because some of the data was varied compared to other groups, there may of have been an error with the liver itself. While the pure samples of liver were the same surface area, not all pieces were blended from the same section of the