Preview

Lab Report Osmosis

Better Essays
Open Document
Open Document
1689 Words
Grammar
Grammar
Plagiarism
Plagiarism
Writing
Writing
Score
Score
Lab Report Osmosis
The effect of osmosis on artificial cells with different concentrations of sucrose

Alex McRae
Biology 120-902 Grand Valley State University 1 Campus Drive Allendale, MI 49401 mcraeal@mail.gvsu.edu

Abstract In this study, we tested the validity of osmosis in artificial animal cells. Osmosis is the diffusion of free water across a membrane. The purpose of the study was to calculate the rate of osmosis in artificial cells containing different concentrations of sucrose and water. We studied the rate of osmosis in artificial cells by creating five different dialysis bags with different concentrations of both sucrose and water and calculating the cumulative change in weight ever 10 minutes for 90 minutes. Our results for the artificial cells showed different concentrations moved from high to low concentrations- through hypotonic movement or hypertonic movement.
Introduction
The main purpose of this paper is to assess the rate of change with osmosis for different concentrations of sucrose in artificial cells. Since the human body is composed of trillions of cells that contain roughly 85% of water, makes osmosis a very important concept (Carmichael, Grabe and Wenger). The forces that affect osmosis are the concentrations of solutes surrounding the cell or inside of the cell. Water will then move across the cell membrane and create a balance of water between the cell and its environment (Reece et al. 133). In order to calculate the average rate of change for our artificial cells, we must understand tonicity as the ability of a nearby solution to cause a cell to lose or gain water, depending on its concentration of non-penetrating solutes relative to solutes inside the cell (Reece et al. 133). The dialysis bags used in this experiment have membranes which are selectively permeable, which only allows particles specifically small enough to pass through (Carmichael, Grabe and Wenger). In a hypotonic solution, water



Cited: Carmichael, Jeff, Mark Grabe and Jonathan Wenger. Biology 150 Laboratory Review. University of North Dakota, n.d. Web. 7 Oct. 2011. Patlak, Joseph and Chris Watters. Diffusion and Osmosis. University of Vermont and Middlebury College, 1997. Web. 8 Oct. 2011. Reece, Jane B., et al. Campbell Biology. San Francisco: Pearson Education Inc., 2005. Print.

You May Also Find These Documents Helpful

  • Good Essays

    The purpose of this lab was to explore the concept of Osmosis using dialysis tubing and different concentrations of sucrose solutions, and to help one better understand what happens to cells when they are exposed to solutions of differing tonicities.…

    • 977 Words
    • 4 Pages
    Good Essays
  • Good Essays

    Ap Bio Lab Report Osmosis

    • 850 Words
    • 4 Pages

    of dialysis tubing, 25 mL of sucrose solutions with concentrations of 0.02 Molar, 0.04M, 0.06M, 0.08M, 1.0M, as well as 25mL of distilled water, six 250 mL beakers, a balance, and paper towels. We first cut the dialysis tubing into 6 pieces, each 1ft. long, and placed them into a beaker of water. We then tied off the dialysis tubing and poured 25 mL of distilled water in. We repeated this with the rest of the five pieces dialysis tubing, pouring a different molarity of sucrose solution in different dialysis tubes. After all the tubes were filled and tied, we then dried the bags and weigh each one on the scale. After all the data was recorded, we filled all the beakers about ¾ full of distilled water, placed the bags into each beaker in unison, and waited 30 minutes. Next, the bags were removed from the beakers, dried, and weighed separately. We expected the mass to increase with increasing molarity because with the higher the concentrations, more water would need to be diffused into the bag to reach…

    • 850 Words
    • 4 Pages
    Good Essays
  • Good Essays

    The objectives of this experiment are to determine the water potential of apple cells and to better understand the movement of water through cells. This will be done by putting cores of apple tissue in sucrose solutions of different concentrations and measuring the changes in mass of the apple cores. The changes in mass reflect the movement of water into or out of the tissue. After determining the concentration of sucrose solution at which there is no net gain or loss of water from the apple tissue, the osmotic potential can be calculated. This will then tell the water potential of the apple cells.…

    • 1050 Words
    • 5 Pages
    Good Essays
  • Powerful Essays

    The water absorption mechanism relies mainly on the change in osmolarity between the extracellular fluids and intracellular fluids. This is…

    • 1776 Words
    • 8 Pages
    Powerful Essays
  • Good Essays

    Osmosis is of great importance in biological processes where the solvent is water. The transport of…

    • 385 Words
    • 2 Pages
    Good Essays
  • Powerful Essays

    Kidney Beans Lab Report

    • 2700 Words
    • 11 Pages

    Cell osmotic concentration: The greater the concentration of soluble compounds, the greater the attraction for water.…

    • 2700 Words
    • 11 Pages
    Powerful Essays
  • Satisfactory Essays

    Open-ended Lab: Osmosis and Diffusion in Dialysis Tubing Name(s): Background: It is very difficult to measure or see osmosis actually occurring in cells because of the small size of most cells. However, if an artificial membrane that acts in some ways like a real plasma membrane could be found, than…

    • 447 Words
    • 2 Pages
    Satisfactory Essays
  • Good Essays

    The purpose of this lab was to observe passive transport of molecules through diffusion and osmosis. We had to keep close observation on two dialysis bags and explain how we measured the change in weight as diffusion and osmosis occurred throughout the experiment.…

    • 533 Words
    • 3 Pages
    Good Essays
  • Better Essays

    Dialysis Lab Report

    • 1202 Words
    • 5 Pages

    Analysis During the experiment, a dialysis bag was used to mimic a cell membrane. Two ways why this model realistically represents a cell is because the dialysis bag is semi-permeable, like a cell. The bag was able to allow water in, as the size of the water molecule was small enough to be able to fit through the pores of the bag. Sucrose was not able to pass through as the molecules were too big to enter.…

    • 1202 Words
    • 5 Pages
    Better Essays
  • Good Essays

    Osmosis Lab Report

    • 839 Words
    • 4 Pages

    The aim of this experiment was to verify the concept of Osmosis and Diffusion with a semi-permeable membrane (dialysis tubing), it will be exposed to different environments and concentration gradients.…

    • 839 Words
    • 4 Pages
    Good Essays
  • Good Essays

    Egg Osmosis

    • 548 Words
    • 3 Pages

    Purpose: We are taking a large single cell (the egg) and demonstrating Osmosis. Osmosis is a type of diffusion where the water molecules is the solution that is being moved. Osmosis is the movement of water through a semi-permeable membrane. Diffusion is the movement of molecules from an area of high concentration to an area of lower concentration.…

    • 548 Words
    • 3 Pages
    Good Essays
  • Good Essays

    The phenomenon being investigated in the lab was how the concentration gradient affects the rate of osmosis in a cell. Osmosis is the passive movement of water from and area of high concentration, temperature, and pressure. The concentration gradient is the difference in concentration though a solution (Concentration Gradient 2012) They all affect the rate of osmosis by increasing the gradient of the solution, and increasing the energy in the system. For this experiment, it is predicted that an increase in solution concentration will cause a proportional change in the rate of osmosis. Creating artificial cells that contained different concentrations of sucrose tested this hypothesis. In ten-minute increments, the weights of the cells were recorded. Overall a change in weight was found and also the slope to show how much the concentration gradient had on the rate of osmosis.…

    • 617 Words
    • 3 Pages
    Good Essays
  • Good Essays

    The cell is the basic unit of living things, and is made up of multiple organelles. Organelles are membrane bound subdivisions, each specialised for a specific function. This experiment looks at the Plasma Membrane, which is a semipermeable layer surrounding the cell. It’s primary job is to control what goes in and out of the cell. Molecules can move across this membrane in either an active movement or a passive movement. Active being that which requires energy and only occurs when molecules are required to move against the concentration gradient. The concentration gradient refers to the difference in the amount of solutes in the solutions of two adjacent areas. This investigation looks more into passive movement rather than active movement and even further into osmosis rather than diffusion. Osmosis is the movement of water across a semipermeable membrane and can only occur when the solute (in this case salt) cannot pass through the membrane. The water molecules move from a region of high solute concentration to an area of low solute concentration, therefore moving down the concentration gradient (refer to Figure 1). The osmotic potential of a cell is it’s capacity to loose water molecules. When the amount of solute in each solution is the same and the amount of molecules moving in and out of a solution is the same, it is known as dynamic equilibrium. When a cell is placed in a solution that is more concentrated than the cell, the cell loses water and therefore has a high osmotic potential. When a cell is placed in a solution that is less concentrated than the cell, the cell gains water and therefore has a low osmotic potential. For example; when a sponge is placed in a cup of cordial solution, the sponge will soak up the solution. In this case, the sponge is acting as the semipermeable membrane and is letting the water molecules in. This happens because the sponge is less concentrated…

    • 1337 Words
    • 6 Pages
    Good Essays
  • Good Essays

    Osmosis Lab

    • 1455 Words
    • 6 Pages

    isThe effect of sucrose concentration on the rate of osmosis across a potato’s cell membrane submerged for 94 hours in the solutation.…

    • 1455 Words
    • 6 Pages
    Good Essays
  • Powerful Essays

    Osmosis Essay

    • 1919 Words
    • 8 Pages

    Abstract - The experiments done in class served for multiple purposes. It helped identify that diffusion is the shifting of molecules from one part to another that takes no energy, and that osmosis is just the flow of water through the cell membrane. The main purpose of the first experiment was to examine the movement of H2O and other materials in living and simulate systems. This was done through the dialysis tubing to mimic the cell membrane and measure the change of mass inside the tube filled with distilled water. The quantity of mass before and after was measured and saw the amount gained from the outside environment (either saline, sucrose, or water) of the cell. Also determined the amount of sucrose in the potato within the second experiment.…

    • 1919 Words
    • 8 Pages
    Powerful Essays