Isolation and Characterization Phytase Producing Microbes from Poultry Soil Sample
Global J. of Mod. Biol. & Tech., 2012: 2 (3) Research Paper: Arunkumar et al., 2012: Pp.68-72. ISOLATION AND CHARACTERIZATION PHYTASE PRODUCING MICROBES FROM POULTRY SOIL SAMPLE Arunkumar.R., Bharath Chandra Sugunesh. G and A.M. Vinothkumar Dept. of Biotechnology, Prathyusha Institute of Technology and Management, Thiruvallur-25. ABSTRACT Phytase producing thirteen microbial strains were identified in poultry soil sample collected at Sathyamangalam, Erode district, Tamilnadu. Among thirteen isolates, two of them were the best of phytases enzyme producer and identified as Aspergillus fumigatus and Aspergillus terreus. Under solid substrate culture condition Aspergillus fumigatus was screened for phytase production on agricultural by-products. The results showed that wheat bran with sugarcane molasses was the best substrate for production of phytase. Phytase purified by non chromatography method, the purified enzyme has an apparent molecular weight 64kDa. Keywords: Phytase, Aspergillus fumigatus and Aspergillus terreus. INTRODUCTION Phytase is widespread in nature, occurring in plants, microorganisms, as well as in some animals. Microbial Phytase activity was most frequently detected in fungi. Phytic acid was discovered as early as in 1872 by Pfeffer and a first note on Phytase can be found in the literature in 1907. Phytase belongs to the group of phosphoric monoester hydrolases; it catalyzes the hydrolysis of myo-inositol hexakisphosphate to inorganic monophosphate and lower phosphoric esters of myo-inositol, or in some cases to free myo-inositol (Vats and Banerjee, 2004 and Howson and Davies, 2005). Shieh and Ware (1968) isolated 30 microorganisms from soil for extracellular Phytase activity. All Phytase producers were filamentous fungi, 28 of them belonged to the genus Aspergillus, one species belonging to Penicillium and on to Mucor. Most of the isolates produced only intracellular Phytase (Ghada et al., 2011). Similarly A. niger strains was best producers of
References: Global J. of Mod. Biol. & Tech., 2012: 2 (3) Fig 1. Phytase Producing organisms Fig 2: Morphology of Phytase producing strains isolated from poultry soil sample Aspergillus terreus Aspergillus fumigatus Fig 3: Three-phase portioning Global J. of Mod. Biol. & Tech., 2012: 2 (3) Table 2: Screening of Media for Aspergillus fumigates S.No Organism Combination Day Phytase activity (nkat) 150.312 6th 197.52 267.38 Extracellular protein (mg) 89 235 301 Biomass (gm) 0.35 0.45 0.62 1 2 3