Immunology Lab Report
Radial Immunodiffussion (RID)
Christian Crespo
18 October 2013
Immunology
Lab Report
Purpose of the Experiment: The objective of this experiment is to quantitatively observe the foundational reaction in our Immune system; the Antigen-Antibody interactions. The Ouchterlony procedure is what will be used in this lab to detect nature of the antibody interaction. The orientations of the band will provide more information about the interaction of antibody and antigen.
Hypothesis:
For this experiment, antibodies will be placed in wells on an agar plate, surrounded by wells containing serum (proteins, antigens etc.). If the antibody interacts with the antigen of surrounding wells, then the presence …show more content…
In this interaction, the antibodies will bind specifically to the epitopes of an antigen that it is specific for. The epitopes that are found on the antigen are also known as antigenic determinants. There are two types of antigens: 1) Particulate antigens & 2) Soluble antigens. In this experiment, the antigen reacting with the antibody was observed to be a soluble antigen. When the soluble antigen binds to antibody it will form antigen-antibody complex. As a result to this binding and forming of the complex, the complex will precipitate in the gel which will be visible to the naked eye (“Antigent-Antibody Interaction,” 4). This formation of a precipitate will allow one to characterize these interactions and make conclusions. However, it is important to consider some limitations in receiving a precipitate in the forming of this complex. The “visible precipitation occurs in the region of equivalence, whereas no visible precipitate forms in regions of antibody or antigen excess ("Ouchterlony test", p. 1). Therefore, when you observe a faint precipitate or no precipitate, this is because you do not have enough antigens for antibody to bind to or the latter. When the antibodies are placed in well A, they will diffuse in the agar gel toward the antigen and the antigen vice versa. There are three main results that will be observed in this experiment: pattern of identity, pattern of partial identity,
Christian Crespo
18 October 2013
Immunology
Lab Report
Purpose of the Experiment: The objective of this experiment is to quantitatively observe the foundational reaction in our Immune system; the Antigen-Antibody interactions. The Ouchterlony procedure is what will be used in this lab to detect nature of the antibody interaction. The orientations of the band will provide more information about the interaction of antibody and antigen.
Hypothesis:
For this experiment, antibodies will be placed in wells on an agar plate, surrounded by wells containing serum (proteins, antigens etc.). If the antibody interacts with the antigen of surrounding wells, then the presence …show more content…
In this interaction, the antibodies will bind specifically to the epitopes of an antigen that it is specific for. The epitopes that are found on the antigen are also known as antigenic determinants. There are two types of antigens: 1) Particulate antigens & 2) Soluble antigens. In this experiment, the antigen reacting with the antibody was observed to be a soluble antigen. When the soluble antigen binds to antibody it will form antigen-antibody complex. As a result to this binding and forming of the complex, the complex will precipitate in the gel which will be visible to the naked eye (“Antigent-Antibody Interaction,” 4). This formation of a precipitate will allow one to characterize these interactions and make conclusions. However, it is important to consider some limitations in receiving a precipitate in the forming of this complex. The “visible precipitation occurs in the region of equivalence, whereas no visible precipitate forms in regions of antibody or antigen excess ("Ouchterlony test", p. 1). Therefore, when you observe a faint precipitate or no precipitate, this is because you do not have enough antigens for antibody to bind to or the latter. When the antibodies are placed in well A, they will diffuse in the agar gel toward the antigen and the antigen vice versa. There are three main results that will be observed in this experiment: pattern of identity, pattern of partial identity,