How to Carry Out Aseptic Techniques in a Batch Culture and in the Laboratory
How to Carry Out Aseptic Techniques in a Batch Culture and in the Laboratory
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| |The batch vessel should be sterilised beforehand using steam. The nutrient medium that is added to the vessel | |
| |should be pre-sterilised using heat and pressure. All the vessel openings should have filters to prevent microbes| |
| |from entering. Suitable pH, temperature and oxygen levels must be provided for the growth of the microbe of | |
| |interest. Aeration can be forced using a sparger, which may be used to improve microbe-nutrient mixing. A | |
| |stirring paddle may also be used. The addition of acid/alkali is used, together with feedback from temperature | |
| |probes in order to control pH at optimum levels. Temperature can be maintained at optimum by the removal of | |
| |excess heat - produced by the microbes - using a cooling water jacket. | |
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| |Within the laboratory all apparatus should be sterilised before use, either by autoclaving glass vessels or by | |
| |gamma-radiation of plastic consumables. Autoclaving heats equipment to 121ºC under pressure. Before transferring | |
| |the culture, the mouth of the bottle should be flamed and the instrument being used for the transfer should be | |
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| | | |
| |The batch vessel should be sterilised beforehand using steam. The nutrient medium that is added to the vessel | |
| |should be pre-sterilised using heat and pressure. All the vessel openings should have filters to prevent microbes| |
| |from entering. Suitable pH, temperature and oxygen levels must be provided for the growth of the microbe of | |
| |interest. Aeration can be forced using a sparger, which may be used to improve microbe-nutrient mixing. A | |
| |stirring paddle may also be used. The addition of acid/alkali is used, together with feedback from temperature | |
| |probes in order to control pH at optimum levels. Temperature can be maintained at optimum by the removal of | |
| |excess heat - produced by the microbes - using a cooling water jacket. | |
| | | |
| |Within the laboratory all apparatus should be sterilised before use, either by autoclaving glass vessels or by | |
| |gamma-radiation of plastic consumables. Autoclaving heats equipment to 121ºC under pressure. Before transferring | |
| |the culture, the mouth of the bottle should be flamed and the instrument being used for the transfer should be | |
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