Preview

How Does Caffeine Affect Zebrafish

Good Essays
Open Document
Open Document
552 Words
Grammar
Grammar
Plagiarism
Plagiarism
Writing
Writing
Score
Score
How Does Caffeine Affect Zebrafish
Nicoleta Nedelcu
Caffeine-induced effects on heart rate in zebrafish embryos and possible mechanisms of action
Observation
Caffeine is found in coffee, tea leaves, cocoa beans, and kola nuts. It is used in prescription and over the counter drugs. It increases blood pressure, mental alertness, and gastrointestinal motility. Cardiovascular development with caffeine and other small molecules can be studied relatively easy using zebrafish. The cardiovascular system is complete in two days after fertilization.
Hypothesis
Methods
1. 1-25 mm of caffeine was injected in the yolk sacs of embryos 2-3 days after fertilization.
2. Adult fish were kept in individual tanks with sponge filters, at 28.5oC, under a 14 hour light and 10 hour dark cycle, and …show more content…
Those embryos with 1 mm of caffeine immediately had decreased heart rates and it continued to decrease for 70 minutes, then the heart rate was stable for 100 minutes, and then it sharply dropped and stopped after 27 minutes. Those embryos with 4 mm f caffeine has similar starting reaction for the first 10 minutes, then the heart rate was stable for the next 61 minutes, then it stopped after 1 minute. Those embryos with 8 mm of caffeine had similar reactions but the heart rate stopped after 44 minutes. Those with 10 mm of caffeine ended at 20 minutes, those with 12 mm of caffeine ended at 16 minutes. And those with 25 mm of caffeine stopped at 14 …show more content…
Critique It doesn’t specify what the hypothesis is which is not good, but the procedures were very detailed. They had control groups for when they used the calcium, but it doesn’t say anything about a control group when they used the caffeine. I like that they used different concentrations of caffeine but I would have liked for them to record the heart rate for an embryo without caffeine. Even though they did not have as many control groups as I would have wanted they were able to prove that the increase in the concentration of caffeine results in decreased heart rate and early death.
Word Cite
Rana, Neha, et al. "Caffeine-induced effects on heart rate in zebrafish embryos and possible mechanisms of action: an effective system for experiments in chemical biology." Zebrafish, vol. 7, no. 1, 2010, p. 69+. Academic OneFile, libproxy.gc.maricopa.edu/login?url=http://go.galegroup.com/ps/i.do?p=AONE&sw=w&u=mcc_glendale&v=2.1&it=r&id=GALE%7CA226047143&sid=summon&asid=21014119af311f6d02883e0c8148edf4. Accessed 4 Apr.
Continue Reading
View Writing Issues

You May Also Find These Documents Helpful

  • Better Essays

    Daphnia Heart rate - Lab Report Essay Example
    • 963 Words
    • 4 Pages

    Daphnia Heart rate - Lab Report Essay Example

    Aim: To see the effect of caffeine on the heart rate of the water flea Daphnia.…

    • 963 Words
    • 4 Pages
    Better Essays
    Read More
  • Good Essays

    Daphnia Lab
    • 1155 Words
    • 5 Pages

    Daphnia Lab

    The purpose of this lab is to test what effect of Nicotine, Acetylcholine, Epinephrine, Caffeine, and Ethanol on an organism’s heart rate.…

    • 1155 Words
    • 5 Pages
    Good Essays
    Read More
  • Good Essays

    The Effect of Caffeine 1% and 2% on Daphnia magna’s Heart Rate
    • 1014 Words
    • 3 Pages

    The Effect of Caffeine 1% and 2% on Daphnia magna’s Heart Rate

    Daphnia consists of Antennae and eye in the head, and heart, brood pouch, eggs, ovary, intestine, and anus. There will be a use of Caffeine with concentration of %1, %2. Caffeine is a stimulant. It will make the nervous system to work faster, and it will cause the blood vessels to shrink. Both of these factors will increase the heart rate of an animal that being investigated. A Microscope will be used. A Microscope is a device that can zoom the species that being under investigated thousands of times, and it has many different lenses. My prediction for this experiment is that caffeine increases the heart beat per…

    • 1014 Words
    • 3 Pages
    Good Essays
    Read More
  • Powerful Essays

    daphina and coffein effect on its heart rate
    • 1395 Words
    • 6 Pages

    daphina and coffein effect on its heart rate

    Caffeine is a bitter, white crystalline xanthine alkaloid that is a psychoactive stimulant drug. Caffeine is found in varying quantities in the beans, leaves, and fruit of some plants, where it acts as a natural pesticide that paralyzes and kills certain insects feeding on the plants. It is most commonly consumed by humans in infusions extracted from the cherries of the coffee plant and the leaves of the tea bush, as well as from various foods and drinks containing products derived from the kola nut. In humans, caffeine has a stimulant effect, causing increased amounts of stimulatory neurotransmitters to be released. If the consumption of caffeine is…

    • 1395 Words
    • 6 Pages
    Powerful Essays
    Read More
  • Good Essays

    Daphnia Experiment
    • 654 Words
    • 3 Pages

    Daphnia Experiment

    In this laboratory experiment, our control was the Daphnia that gets spring water, whereas the drugs were the independent variable and the heart rate was the dependable variable. The first step our group did was to place the Daphnia into a clean depression slide. Next, we added a small drop of the spring water and put a cover slip over the slide. Then, we waited for one minute prior to placing the slide on the microscope in order to allow time for either solution or the drug to take its effect. After we put the slide on the microscope, we located the heart of Daphnia and counted its heartbeats while the other member kept time for each three 10-second trials. We recorded our data and calculated the average heart rates of three trials we found during the experiment. Afterwards, we calculated the average 60-second heart rates by multiplying the averages of our 10-second heart rates by 6. These procedures were repeated with the rest of solutions as well, including caffeine, aspirin, alcohol, sleep-aid, and…

    • 654 Words
    • 3 Pages
    Good Essays
    Read More
  • Good Essays

    Variegatus Lab
    • 882 Words
    • 4 Pages

    Variegatus Lab

    Ethanol was given at 0.1mM and 1.0mM. The pulse rate difference was 10.31. Caffeine was given at 0.1mM and 5mM. The pulse rate difference was 1.7241. Since the worms were small and weighed little, they absorbed the drugs at a faster rate. Within 5 minutes the drugs had affected their original heart rate. The worm’s size and the worm’s heart rate work together. This experiment is valid because the worms at first we’re more active and after the drug was administered they became more still. With a higher concentration of alcohol the worms’ ends began to curl while their bodies stayed straight. For the most part, their pulsation rates decreased because alcohol is a depressant. Alcohol causes the organisms to feel sluggish and if a higher dosage was administered to the worms neurological damage could have occurred. The findings are reliable because those that also used alcohol with the worms conducted similar findings. With a smaller dose of ethanol, the change in pulse rates from initial heart rate to final heart rate was more in the negatives. Meanwhile, caffeine is an excitatory drug and causes organisms to experience bursts of energy. Caffeine is an antagonist, which means that it occupies receptors without activating them. It caused the worms to curl up and stretch, as their pulses increased. Then nicotine caused the worms to become more…

    • 882 Words
    • 4 Pages
    Good Essays
    Read More
  • Good Essays

    Daphni Conclusion
    • 455 Words
    • 2 Pages

    Daphni Conclusion

    According to the experiment, the average normal heart rate of Daphnia is 20 heart beats per 12.94 seconds. There were six total trials done for Normal Heart Rate with 2 Daphnia (3 Trials each). The aim of this experiment was to measure the effect of neurotransmitters on the heart rate of Daphnia. When the adrenaline was added to the liquid around Daphnia and after a few seconds, its heart rate sped up. For example, during the first trial, the heart rate went from 20 H.B. per 12.54 seconds (For Normal) to 20 H.B. per 8.99 seconds after adrenaline was added. The results were the same during the last trial also. E.g. the normal heart rate was 20 H.B. per 15.38 seconds while the heart rate after adrenaline was added was 20 H.B. per 6.70 seconds. This proves that adrenaline excites the neurotransmitters and causes the heart rate to speed up.…

    • 455 Words
    • 2 Pages
    Good Essays
    Read More
  • Better Essays

    Daphnia Research Paper
    • 2466 Words
    • 10 Pages

    Daphnia Research Paper

    By a different mechanism (that doesn't involve beta-1 adrenoceptors), caffeine also increases the amount of cAMP in the sinoatrial node. Then cAMP levels increase and this increases the electrical activity of the sinoatrial node, making it depolarize and 'beat' faster. Caffeine has additional effects on the heart. Like adrenaline and noradrenaline, it can affect the main pumping chambers (ventricles) leading to an increase in the rate of contraction and relaxation of each heart beat. This means that as well as beating faster, the heart's individual beats are associated with an increased volume of blood ejected into the circulation per unit time. This is called increasing cardiac output. Two or three cups of strong coffee or tea contain enough caffeine (and a similar acting compound called theobromine) to cause an increase in human heart rate of 5-20 beats/min.…

    • 2466 Words
    • 10 Pages
    Better Essays
    Read More
  • Better Essays

    short lab report bio 102
    • 1527 Words
    • 10 Pages

    short lab report bio 102

    In order to understand a little bit about the effects of common household drugs such as nicotine and caffeine on pulsation rate, an experiment was conducted with Lumbriculus Variegattus (Blackworms). In week 1, we observed the basal rate of three different worms, and then placed the same three worms on different concentrations of caffeine and nicotine. “Caffeine is known to act as an inhibitory neurotransmitter in the brain; it promotes sleep and suppresses aurosal, binding to the adenosine neurotransmitter receptor to block its inhibitory action” (CCNY lab manual, 2014). Before our experiment was conducted we hypothesized that increasing the concentration of caffeine will increase the pulsation rate. During this experiment we used 1.0mM, 3.0mM, and 10.0mM concentrations of caffeine. The highest the concentration the more effect it would have on the pulsation rate, so in this case we could assume that 10.0mM would have the highest effect on the pulsation rate of the blackworms.…

    • 1527 Words
    • 10 Pages
    Better Essays
    Read More
  • Satisfactory Essays

    Snab
    • 401 Words
    • 2 Pages

    Snab

    In this experiment we were examining the effect of caffeine on the heart rate of Daphnia which are water fleas. In my opinion I think that the caffeine will affect the heart rate because when the caffeine enters your blood from the stomach and small intestine it begins to stimulate your central nervous system, the Caffeine stimulates receptors in the cells of your heart to increase your heart rate. The increase of this heart rate speeds up your blood flow as well as increase in blood sugar, urination and blood sugar. So, considering that you can see the through the transparent fleas after dropping the caffeine on them you will witness the hearts beat as a greater haste than usual. So, I will experiment this hypothesis by placing Daphnia under a microscope and recording the heartbeats before pouring the caffeine and after pouring the caffeine.…

    • 401 Words
    • 2 Pages
    Satisfactory Essays
    Read More
  • Good Essays

    Zebrafish Development
    • 1389 Words
    • 6 Pages

    Zebrafish Development

    Using genes that control development makes it possible to identify mutants that alter development. Anterior –Posterior axis formation is controlled by protein gradients in the early embryo development. Bicoid and nanos mRNAs are both maternally provided and are localized to opposite poles of the embryo (Springfield, 2016). Bicoid proteins are concentrated at the anterior end and nanos proteins concentration is highest at the posterior end of the embryo (Springfield, 2016). The combination of how much Bicoid protein and how much Nanos protein a cell receives coordinates the axis formation (Springfield, 2016). If this pattern is altered than mutation rise by causing different expected phenotypes.…

    • 1389 Words
    • 6 Pages
    Good Essays
    Read More
  • Satisfactory Essays

    Health Care
    • 279 Words
    • 2 Pages

    Health Care

    Information I will provide in this report will show whether consuming coffee increases a person’s heart rate.…

    • 279 Words
    • 2 Pages
    Satisfactory Essays
    Read More
  • Powerful Essays

    Pain Perception Research Paper
    • 2861 Words
    • 12 Pages

    Pain Perception Research Paper

    (1996). Effects of caffeine on intracellular calcium release and calcium influx in a colonal cell line. Life Science, v.58, n.12, p.983-990.…

    • 2861 Words
    • 12 Pages
    Powerful Essays
    Read More
  • Powerful Essays

    Cell Cycle Regulation - Genetics
    • 1375 Words
    • 6 Pages

    Cell Cycle Regulation - Genetics

    Cells with mutations in this checkpoint pathway or cultured mammalian cells treated with caffeine will proceed through mitosis with unreplicated DNA.…

    • 1375 Words
    • 6 Pages
    Powerful Essays
    Read More
  • Good Essays

    The Zebrafish Model
    • 1159 Words
    • 5 Pages

    The Zebrafish Model

    The zebrafish shares roughly 70% of its genes with humans, which makes zebrafish an important tool in the discovery of the pathways of diseases, as well as the discovery of genes associated with diseases. The neural circuitary in the brain is an important feature in the development of humans, and in zebrafish, this can be intricately examined, specifically in the case of locomotion. When touch evoked response is initiated, similarly to humans it can be altered, and as the neural circuit is more simple in zebrafish, it provides with an important research tool to the study of human locomotive diseases. [2] Zebrafish experimentation is also cheap, the Zebrafish produces hundreds of eggs at a time, as well as year round, and they require very little maintenance, so these experiments can be done quickly and easily too.…

    • 1159 Words
    • 5 Pages
    Good Essays
    Read More

Related Topics