Genetic Engineering Tutorial
Chapter 14 Genetic Engineering
Choose the best answer for each question.
1. Using this key, put the phrases in the correct order to form a plasmid carrying the recombinant DNA.
Key: 1) use restriction enzymes 2) Use DNA ligase 3) Remove plasmid from parent bacterium 4) Introduce plasmid into new host bacterium.
A. 1, 2, 3, 4 C. 3, 1, 2, 4
B. 4, 3, 2,1 D. 2, 3, 1, 4
2. Which is not a clone? A. a colony of identical bacterial cells B. identical quintuplets C. a forest of identical trees D. eggs produced by oogenesis E. copies of a gene through PCR
3. Restriction enzymes found in bacterial cells are ordinarily used A. during DNA replication B. to degrade the bacterial cell’s DNA C. to degrade viral DNA that enters the cell D. to attach pieces of DNA together
4. Recombinant DNA technology is used A. for gene therapy B. to clone a gene C. to make a particular protein D. to clone a specific piece of DNA E. All of these are correct
5. In order for bacterial cells to express human genes, A. the recombinant DNA must not contain introns. B. reverse transcriptase is sometimes used to make complementary DNA from an mRNA molecule. C. bacterial regulatory genes must be included. D. All of these are correct.
6. The polymerase chain reaction A. utilizes RNA polymerase B. takes place in huge bioreactors C. utilizes temperature insensitive enzyme D. makes lots of nonidentical copies of DNA E. All of these are correct
7. DNA fingerprinting can be used for which of these? A. identifying human remains B. identifying infectious diseases C. finding evolutionary links between organisms D. solving crimes E. All of these are correct
8. DNA amplified by PCR and then used for fingerprinting could come from A. any diploid or haploid cell B. only white blood cells that have been
Choose the best answer for each question.
1. Using this key, put the phrases in the correct order to form a plasmid carrying the recombinant DNA.
Key: 1) use restriction enzymes 2) Use DNA ligase 3) Remove plasmid from parent bacterium 4) Introduce plasmid into new host bacterium.
A. 1, 2, 3, 4 C. 3, 1, 2, 4
B. 4, 3, 2,1 D. 2, 3, 1, 4
2. Which is not a clone? A. a colony of identical bacterial cells B. identical quintuplets C. a forest of identical trees D. eggs produced by oogenesis E. copies of a gene through PCR
3. Restriction enzymes found in bacterial cells are ordinarily used A. during DNA replication B. to degrade the bacterial cell’s DNA C. to degrade viral DNA that enters the cell D. to attach pieces of DNA together
4. Recombinant DNA technology is used A. for gene therapy B. to clone a gene C. to make a particular protein D. to clone a specific piece of DNA E. All of these are correct
5. In order for bacterial cells to express human genes, A. the recombinant DNA must not contain introns. B. reverse transcriptase is sometimes used to make complementary DNA from an mRNA molecule. C. bacterial regulatory genes must be included. D. All of these are correct.
6. The polymerase chain reaction A. utilizes RNA polymerase B. takes place in huge bioreactors C. utilizes temperature insensitive enzyme D. makes lots of nonidentical copies of DNA E. All of these are correct
7. DNA fingerprinting can be used for which of these? A. identifying human remains B. identifying infectious diseases C. finding evolutionary links between organisms D. solving crimes E. All of these are correct
8. DNA amplified by PCR and then used for fingerprinting could come from A. any diploid or haploid cell B. only white blood cells that have been