Gene Expression with E.Coli
Title Gene expression with E.coli bacteria through means of transformation with plasmid DNA
Abstract Science has discovered that with gene expression and genetic engineering, DNA and organisms can be manipulated like never before. This has become an extraordinary discovery because it has lead us to countless medicinal products and cures for diseases and continues to serve as a great asset as research continues. This lab consisted of introducing a plasmid that contains an ampicillin-resistance gene into the common bacteria E.coli. With this, transformation is expected to occur and demonstrate how this prokaryotic, E.coli, uptakes a plasmid that allows it to become resistant to antibiotics and with this information, scientists will be able to use it for agricultures, vaccines, biodegradable products and so on.
The experimental design allowed for the lab to be split up into two groups of plasmid. One of the groups had the “Control plasmid DNA” while the other had the “Plasmid lux DNA” and both group contained a No plasmid used as a negative control. A series of step such as ice baths and heat shocks were put into place to allow for transformation to occur and then the solutions were placed on an agar plate and incubated at 37° for a week. Our results failed to reject our hypothesis and predictions and thus resulted that there was indeed a high percentage of transformation efficiency. The agar plate with the ampicillin environment but no plasmid uptake resulted in no growth while the rest that were a variation between ampicillin environment and nutrient broth have both lawn growth and colonial growth.
Introduction
When talking about DNA and gene expression, let’s admit that there is tons to talk about. With proteins being the building blocks of life that are responsible for determining the chemical and physical structure of a cell or organism, it must be kept in mind that these molecules grabbed this
Abstract Science has discovered that with gene expression and genetic engineering, DNA and organisms can be manipulated like never before. This has become an extraordinary discovery because it has lead us to countless medicinal products and cures for diseases and continues to serve as a great asset as research continues. This lab consisted of introducing a plasmid that contains an ampicillin-resistance gene into the common bacteria E.coli. With this, transformation is expected to occur and demonstrate how this prokaryotic, E.coli, uptakes a plasmid that allows it to become resistant to antibiotics and with this information, scientists will be able to use it for agricultures, vaccines, biodegradable products and so on.
The experimental design allowed for the lab to be split up into two groups of plasmid. One of the groups had the “Control plasmid DNA” while the other had the “Plasmid lux DNA” and both group contained a No plasmid used as a negative control. A series of step such as ice baths and heat shocks were put into place to allow for transformation to occur and then the solutions were placed on an agar plate and incubated at 37° for a week. Our results failed to reject our hypothesis and predictions and thus resulted that there was indeed a high percentage of transformation efficiency. The agar plate with the ampicillin environment but no plasmid uptake resulted in no growth while the rest that were a variation between ampicillin environment and nutrient broth have both lawn growth and colonial growth.
Introduction
When talking about DNA and gene expression, let’s admit that there is tons to talk about. With proteins being the building blocks of life that are responsible for determining the chemical and physical structure of a cell or organism, it must be kept in mind that these molecules grabbed this