extraction of DNA
Extraction of DNA
Science Fair Project 2013-2014
Produced by Nadia Walker Due Date: Monday January 20th 2014
Presented to Mr. Freimann Date Submitted: Monday January 20th 2014
Table of Contents
Introduction page 1
Hypothesis and materials page 2
Procedure page 3
Analysis page 4
Conclusion page 5
Introduction
DNA isolation is a process of purification of DNA from sample using a combination of physical and chemical methods. The first isolation of DNA was done in 1869 by Friedrich Miescher. Currently it is a routine procedure in molecular biology or forensic analyses. The process of extracting DNA from a cell is the first step for many laboratory procedures in biotechnology. The scientist must be able to separate DNA from the unwanted substances of the cell gently enough so that the DNA does not break up. In this experiment, 10 different fruits are treated to extract their DNA.
Hypothesis
My hypothesis is that Method 1 will be more effectice than Method 2.
Materials
• 10 different fruits
• Salt
• Soap
• Water
• Kettle
• Ice cubes
• Timer
• Blender
• Thermometer
• Coffee filter
• 99% Rubbing Alcohol
• Test tubes
• Steering tool
• Beakers
• Measuring cup
• Ziplock bags
Procedure
Method 1:
Stage 1- preparing the fruit
• chop fruit in small pieces
• prepare a solution of 10cm3 of
Science Fair Project 2013-2014
Produced by Nadia Walker Due Date: Monday January 20th 2014
Presented to Mr. Freimann Date Submitted: Monday January 20th 2014
Table of Contents
Introduction page 1
Hypothesis and materials page 2
Procedure page 3
Analysis page 4
Conclusion page 5
Introduction
DNA isolation is a process of purification of DNA from sample using a combination of physical and chemical methods. The first isolation of DNA was done in 1869 by Friedrich Miescher. Currently it is a routine procedure in molecular biology or forensic analyses. The process of extracting DNA from a cell is the first step for many laboratory procedures in biotechnology. The scientist must be able to separate DNA from the unwanted substances of the cell gently enough so that the DNA does not break up. In this experiment, 10 different fruits are treated to extract their DNA.
Hypothesis
My hypothesis is that Method 1 will be more effectice than Method 2.
Materials
• 10 different fruits
• Salt
• Soap
• Water
• Kettle
• Ice cubes
• Timer
• Blender
• Thermometer
• Coffee filter
• 99% Rubbing Alcohol
• Test tubes
• Steering tool
• Beakers
• Measuring cup
• Ziplock bags
Procedure
Method 1:
Stage 1- preparing the fruit
• chop fruit in small pieces
• prepare a solution of 10cm3 of