Preview

Exploring enzyme lab report

Good Essays
Open Document
Open Document
1052 Words
Grammar
Grammar
Plagiarism
Plagiarism
Writing
Writing
Score
Score
Exploring enzyme lab report
Exploring Enzymes - Ground-Up Tissue Activity
Abstract
Our experiment looked at how increasing the surface area of a substance affects the amount of bubbles created due to the presence of the enzyme catalase. The experiment used two pieces of fish, one whole and one ground up, which were then covered in hydrogen peroxide. This method allowed us to observe the catalase in ground up fish break down the hydrogen peroxide at a quicker rate than in the piece of fish left intact. This was determined by observing a larger formation of bubbles, the visual indicator of hydrogen peroxide being broken down into a non-toxic form of hydrogen and water. This study allowed us to observe first hand catalase can perform further when the surface area is larger.
Introduction
A majority of individuals do not realize how important enzymes, a type of protein, are for life to exist. Organisms experience a continuum of chemical reactions taking place within their cells and these reactions wouldn’t happen without the presence of enzymes. Since enzymes are so important to life we should understand what affects their productivity to include: temperature, pH and the concentration of reactants. The purpose of this study is to test how the enzymes react when there is a larger surface area; hence an increase in the concentration of reactants.
In this experiment we use the enzyme catalase, which assists in the breakdown of hydrogen peroxide into water and oxygen. Hydrogen peroxide is a well-known chemical used in a variety of ways such as household cleaning, wound management and removing color from a variety of items. Hydrogen peroxide is a product of living cell reactions and is considered deadly and if not disposed of properly a cell killer. Although it sounds like doom and gloom this is where the catalase steps in, an enzyme to breakdown the hydrogen peroxide into the less toxic substances of hydrogen and water.
Hypothesis
If the surface area is increased than the

You May Also Find These Documents Helpful

  • Good Essays

    Enzyme Lab Report

    • 492 Words
    • 2 Pages

    The results of our experiment showed the solutions in both tube 1 and tube 2 increasing in absorbency in the first eight minutes but then tube 1 continued to increase while tube 2 began to balance out. Tube 3, our blank, managed to stay at 0nm the entire twenty minutes. From this data, we can conclude that our hypothesis was supported that EDTA had a greater change in absorption over PTU.…

    • 492 Words
    • 2 Pages
    Good Essays
  • Good Essays

    Catalase Lab Report

    • 563 Words
    • 3 Pages

    Next, 50ml of distilled water will be measured using a graduated cylinder. The 50g of catalase and the 50ml of distilled water will be put into a blender and blended until liquefied. The catalase solution will then be introduced to a Buchner funnel filtration system to separate the catalase extract from any solid particles left over from the blending process. This step will be repeated until all of the catalases have been blended and filtered. Next, 5 ml of hydrogen peroxide will be measured out using a graduated cylinder. It will then be poured into a small Erlenmeyer flask. This will be repeated five times, representing the five trials for each catalase treatment. Once the Erlenmeyer flask has been given 5ml of hydrogen peroxide, 10ml of the S. oleracea catalase will be measured out and poured into the Hydrogen peroxide filled flask. When the catalase and Hydrogen peroxide meet a reaction will occur and the hydrogen peroxide will be broken down into water and oxygen. The oxygen released from the reaction will be recorded by the Gas Pressure sensor. This step will be repeated five times for each necessary trial, then the next catalase extract will be…

    • 563 Words
    • 3 Pages
    Good Essays
  • Good Essays

    Lab 6 enzymes

    • 1000 Words
    • 5 Pages

    Preparation Step 1: Research (online) the reaction between catalase and hydrogen peroxide and be familiar with the reactants, products, and enzyme. You will include the overall reaction in your lab report for this experiment. We will measure enzyme activity by measuring the height of the bubbles produced.…

    • 1000 Words
    • 5 Pages
    Good Essays
  • Satisfactory Essays

    lab 6

    • 798 Words
    • 4 Pages

    1. Research (online) the reaction between catalase and hydrogen peroxide and be familiar with the reactants, products, and enzyme. You will include the overall reaction in your lab report for this experiment. We will measure enzyme activity by measuring the height of the bubbles produced.…

    • 798 Words
    • 4 Pages
    Satisfactory Essays
  • Good Essays

    Peroxidase is an enzyme found in potatoes that catalyzes the breakdown of hydrogen peroxide, H2O2, into O2 gas and water. We examined the different pH environments that can affect the enzyme activity during the breakdown of H2O2. In order to do this, we added different levels of pH, low, medium, and high, into different test tubes with the enzyme and H2O2, and we then inverted the tube. The amount of O2 gas produced was then measured and recorded. The result was that the higher pH produced more gas, followed by medium pH, then low pH. The enzymes were more active in the pH of about 10. It increased the enzyme activity allowing more O2 to be produced from the breakdown of the hydrogen peroxide.…

    • 621 Words
    • 3 Pages
    Good Essays
  • Satisfactory Essays

    Enzyme Lab

    • 353 Words
    • 2 Pages

    The purpose of this lab is to explain how enzymes act as catalysts for biological reactions in different temperatures.…

    • 353 Words
    • 2 Pages
    Satisfactory Essays
  • Good Essays

    Enzyme Lab Report

    • 1060 Words
    • 5 Pages

    Problem: How can we demonstrate how enzymes work? What happens if we alter the environment of an enzyme?…

    • 1060 Words
    • 5 Pages
    Good Essays
  • Better Essays

    Enzymes Lab Report

    • 1657 Words
    • 7 Pages

    In this lab we explore an enzymes activity and how it can be affected by changes to its environment. An enzyme is a protein and is a catalyst to chemical reactions. It helps accelerate reactions by lowering the activation energy, which is needed for reactions in cells to progress at a higher rate. Activation energy is the minimum amount of energy needed for a chemical reaction to occur, yielding products from a given set of reactants. (Unit 7: Enzymes lab)…

    • 1657 Words
    • 7 Pages
    Better Essays
  • Good Essays

    Catalase Lab Report

    • 370 Words
    • 2 Pages

    Purpose: To observe the effects of hydrogen peroxide on living tissue and non-living materials, showing the presence or absence of catalase as observed by the presences of bubbles.…

    • 370 Words
    • 2 Pages
    Good Essays
  • Good Essays

    The purpose of this lab was to compare the action of a catalyst (enzyme) under different environmental conditions. This was determined by performing a variety of different experiments. The first experiment was performed by adding hydrogen peroxide to sand. Due to the fact that the sand was not soluble in the hydrogen peroxide, no reaction thus no catalyst were present. Manganese dioxide was also added to the hydrogen peroxide creating a moderately fast reaction thus leading to believe that an enzyme was present to lower the activation energy and speed up the reaction.…

    • 446 Words
    • 2 Pages
    Good Essays
  • Good Essays

    Enzyme Lab Report

    • 1186 Words
    • 5 Pages

    Enzymes are biological catalysts that lower the amount of activation energy needed in carrying out biochemical reactions1. Enzymes are responsible for almost every reaction that occurs in a cell and is named according to the substrate they specifically affect. An enzyme works best under optimal conditions pertaining to temperature, pH level and salt concentration1. In unfavorable conditions enzymes will become denatured and ineffective. In human beings enzymes are most effective in 37 C temperatures, at a pH level of 7.2 (in the stomach a pH of 2 and in the small intestine a pH of 8.5)3 and with a daily…

    • 1186 Words
    • 5 Pages
    Good Essays
  • Better Essays

    Catalase Enzyme Lab

    • 1489 Words
    • 6 Pages

    Part A: If the liver is placed in 2 ml of 3% hydrogen peroxide, then the result will be a high rate of reaction because catalase reacts with hydrogen peroxide to form hydrogen and oxygen. If pieces of potato, chicken, and apple are placed into 2 ml of hydrogen peroxide separately, then the chicken will have the highest reaction…

    • 1489 Words
    • 6 Pages
    Better Essays
  • Satisfactory Essays

    This experiment will investigate the effect of temperature on the rate of catalase activity by measuring the number of oxygen gas bubbles produced.…

    • 849 Words
    • 4 Pages
    Satisfactory Essays
  • Satisfactory Essays

    Enzyme Lab

    • 338 Words
    • 10 Pages

    In our everyday lives, enzymes are used in our bodies, and in nature around us, to speed up the chemical reactions happening constantly, which happens by lowering the amount of activation energy needed to start various reactions. The way this works is by attaching the particular substrate to the active site of the enzyme, where it will start to aid the chemical reaction. Then, the allosteric site involves itself in forming the final 3D shape. For each specific reaction is a specific enzyme that helps speed up the reaction, and the reason for the variations of the enzymes is their unique protein structures. However, this means that once the structure of the enzyme is denatured and changed, the functions will most probably modify as well. In nature, this happens when the temperature and concentrations of different components are altered. In this lab experiment, we will be doing an in-depth research of exactly what happens to the enzymes, when it happens, and why it denatures the way it does.…

    • 338 Words
    • 10 Pages
    Satisfactory Essays
  • Good Essays

    Ib Biology Enzymes Ia

    • 1408 Words
    • 6 Pages

    Hydrogen peroxide (H2O2) is naturally formed in living organisms, however it is very harmful and is broken down immediately by several enzymes including catalase. This enzyme catalyses the breakdown of hydrogen peroxide to water and oxygen. Persons with acatalasemia (a hereditary condition) have extremely low catalase activity and, although present worldwide, it is more commonly found in Koreans. Hydrogen Peroxide is usually used as a topical disinfectant in wounds and the bubbling that is seen in the experiment is due to the oxygen gas released from the tested substance. Because of this, any cell that uses oxygen or lives in the presence of oxygen must have a way to get rid of the peroxide. One of these ways is to make catalase.…

    • 1408 Words
    • 6 Pages
    Good Essays