Chromatography Lab Repot
Design and carry out a scientific investigation on any one factor that affects retention on paper chromatography
Design and carry out a scientific investigation on any one factor that affects retention on paper chromatography
CHEMISTRY LAB REPORT
CHEMISTRY LAB REPORT
Name: Sri Danielle
Class: 9A
Teacher: Sir Ajay
Name: Sri Danielle
Class: 9A
Teacher: Sir Ajay
Aim
My aim for this experiment is to find out how different types of solvent will affect the retention factor in paper chromatography.
Hypothesis
I think that there will be a high RF value in paper chromatography, if we use water solvent because a solvent that has a strong interaction with a chemical will easily over come attraction from the chemical for the absorbent layer and move that chemical in a given period of time.
Materials
Food Coloring (solute)
Ethanol, Acetone, Tap Water, Distilled Water(solvents)
Pencil
Ruler
Scissors
Filter Paper
Petri Dish
Variables
Independent: Type of chromatography solvent
Dependent: Retention in paper chromatography
Controlled: Type of Chromatography paper Size of spot Thickness of Chromatography Substance tested for paper chromatography Concentration of the solute Temperature
Variables Manipulation
Independent: Apply different solvents
Dependent: The RF value of the given solvent
Controlled: Keep the solute same. Keep the size of the solution the same (one drop) Keep the temperature of the room the same.
Diagram
Method
Step 1 – Prepare Materials and pour solvents into the petri dish.
Step 2- Fold the provided chromatography paper into half (make sure it can stand)
Step 3- measure 1.5cm from one end of the paper then draw a line across. This will be used as a starting point.
Step 4- Repeat for 4 chromatography papers for each solvent.
Step 5- Place a drop of the solution on the pencil line for all 4
Design and carry out a scientific investigation on any one factor that affects retention on paper chromatography
CHEMISTRY LAB REPORT
CHEMISTRY LAB REPORT
Name: Sri Danielle
Class: 9A
Teacher: Sir Ajay
Name: Sri Danielle
Class: 9A
Teacher: Sir Ajay
Aim
My aim for this experiment is to find out how different types of solvent will affect the retention factor in paper chromatography.
Hypothesis
I think that there will be a high RF value in paper chromatography, if we use water solvent because a solvent that has a strong interaction with a chemical will easily over come attraction from the chemical for the absorbent layer and move that chemical in a given period of time.
Materials
Food Coloring (solute)
Ethanol, Acetone, Tap Water, Distilled Water(solvents)
Pencil
Ruler
Scissors
Filter Paper
Petri Dish
Variables
Independent: Type of chromatography solvent
Dependent: Retention in paper chromatography
Controlled: Type of Chromatography paper Size of spot Thickness of Chromatography Substance tested for paper chromatography Concentration of the solute Temperature
Variables Manipulation
Independent: Apply different solvents
Dependent: The RF value of the given solvent
Controlled: Keep the solute same. Keep the size of the solution the same (one drop) Keep the temperature of the room the same.
Diagram
Method
Step 1 – Prepare Materials and pour solvents into the petri dish.
Step 2- Fold the provided chromatography paper into half (make sure it can stand)
Step 3- measure 1.5cm from one end of the paper then draw a line across. This will be used as a starting point.
Step 4- Repeat for 4 chromatography papers for each solvent.
Step 5- Place a drop of the solution on the pencil line for all 4