Cell Membrane Transport
Cell Membrane Transport The purposes for these experiments is to be able to understand osmosis and its relationship to tonicity of solutions, and the transportation of molecules across cell membranes. It's also used to understand, and how temperature affects diffusion. You also learn how to test for presences of starches and sugars in solutions. In the first exercise I will be testing for diffusion through an artificial membranes. This one has forty five steps to it. I will not go into it step by step but I'm going to say just the important ones. You need a cup to put 150mL of distilled water in cup number one. Then you soak the dialysis tubing in this cup for five minutes. Then you add in the graduated cylinder, 4mL of distilled water, 2 mL of starch solution, and 2 mL of the glucose solution, then pour it into cup number two. Then use the glass stirring rod to stir the solution in cup two. Then remove the dialysis tubing from cup one, set the cup aside for later, tie up one end tightly. Then test the tubing with distilled water for any leaks, then pour out the water. Place a funnel at the end of the tubing and then pour the glucose-starch solution into the dialysis tubing from cup two. Then get all of the air out of the tubing and then tie that end securely. Rinse the outside of the tubing to remove what contents could have gotten on the outside of it. Then record the color of solution inside the dialysis tubing. Then use the IKI dropping pipet to slowly add IKI solution to the 150 mL in cup one until it looks like the color of strong tea, stirring with the glass rod while adding the drops. Then record the color of the contents in cup one in before dialysis. Put the dialysis tubing inside cup one, and you let sit for an hour. After the hour, record the color of the dialysis tubing under after dialysis. Then clean cup two and label it dialysis tubing contents. Hold the dialysis tubing over cup two and cut the tubing to release the