Campylobacter Isolation Lab Report
To
My parents, Mr. Therdsak and Mrs. Aree Jainonthee who always behind every success of my life ACKNOWLEDGEMENT
I would like to express my sincere thanks to my thesis advisor, Asst. Prof. Dr. Duangporn Pichpol for her invaluable suggestions and encouragement throughout the period of this research. I am most grateful for her teaching and advice on research methodologies and techniques performed in microbiology testing. Her close attention on my working life and my career path are very much appreciated. I would not have achieved this far and this thesis would not have been completed without all the support that I have always received from her.
I would like to extend my sincere thanks to the initiator of the double international program of Master …show more content…
The samples were firstly isolated using a special filtration technique that allowed only Campylobacter bacteria to pass through and then inoculated on to a selective medium. This first fecal culture was proved that intestinal infection was the origin of Campylobacter bacteremia (Dekeyser, Gossuin-Detrain, Butzler, & Sternon, 1972 (as cited in Butzler, 2004)). A collaboration of Dekeyser and Butzler was the starting point of detection the agent in stools of diarrhea patients. In 1973, first case of Campylobacter enteritis was reported in Africa in Zaire where now known as the Democratic Republic of Congo (Butzler, 1978 (as cited in Butzler, 2004)). Development of new selective media decreased the need of filter suspensions and allowed Campylobacter effortlessly to be isolated (Altekruse et al., 1999). In 1979, the first Campylobacter enteritis in human was fully published (Butzler & Skirrow, 1979 (as cited in Butzler, 2004)). After Kist reported Escherich’s findings at the Third International Campylobacter Workshop in Ottawa in 1985, seemed to be the first finding of Campylobacter in human, nowadays, more cases of Campylobacter infection have been reported throughout the world. The organism is considered to be the major common cause of bacterial gastroenteritis in human (WHO, 2013). Historical timeline of Campylobacter …show more content…
C. jejuni gave positive results to catalase and indoxyl acetate. Most of Campylobacter are susceptible to nalidixic acid and resistant to cephalothin (Barrett, Patton, & Morris, 1988). C. jejuni (subspecies jejuni) and C. coli are the most leading species among Campylobacter in public health concerns that cause gastroenteritis in human (WHO, 2016) and more likely to differentiate the two species with biochemical tests by using hydrolysis of hippurate (International Organization for Standardization [ISO], 2006). Other phenotypic characteristics such as oxidase positive, motility (81%), nitrate reduction, capability to grow on MacConkey’s agar, growth in 1% glycine, and unable to utilize glucose (Hunt, Abeyta, & Tran, 2001). In addition, the thermophilic C. jejuni requires temperature between 37 and 42 oC to grow (Davis & DiRita, 2008) with the optimum temperature at 41.5 oC (ISO, 2006). The bacteria are incapable to grow below 30 oC since cold
My parents, Mr. Therdsak and Mrs. Aree Jainonthee who always behind every success of my life ACKNOWLEDGEMENT
I would like to express my sincere thanks to my thesis advisor, Asst. Prof. Dr. Duangporn Pichpol for her invaluable suggestions and encouragement throughout the period of this research. I am most grateful for her teaching and advice on research methodologies and techniques performed in microbiology testing. Her close attention on my working life and my career path are very much appreciated. I would not have achieved this far and this thesis would not have been completed without all the support that I have always received from her.
I would like to extend my sincere thanks to the initiator of the double international program of Master …show more content…
The samples were firstly isolated using a special filtration technique that allowed only Campylobacter bacteria to pass through and then inoculated on to a selective medium. This first fecal culture was proved that intestinal infection was the origin of Campylobacter bacteremia (Dekeyser, Gossuin-Detrain, Butzler, & Sternon, 1972 (as cited in Butzler, 2004)). A collaboration of Dekeyser and Butzler was the starting point of detection the agent in stools of diarrhea patients. In 1973, first case of Campylobacter enteritis was reported in Africa in Zaire where now known as the Democratic Republic of Congo (Butzler, 1978 (as cited in Butzler, 2004)). Development of new selective media decreased the need of filter suspensions and allowed Campylobacter effortlessly to be isolated (Altekruse et al., 1999). In 1979, the first Campylobacter enteritis in human was fully published (Butzler & Skirrow, 1979 (as cited in Butzler, 2004)). After Kist reported Escherich’s findings at the Third International Campylobacter Workshop in Ottawa in 1985, seemed to be the first finding of Campylobacter in human, nowadays, more cases of Campylobacter infection have been reported throughout the world. The organism is considered to be the major common cause of bacterial gastroenteritis in human (WHO, 2013). Historical timeline of Campylobacter …show more content…
C. jejuni gave positive results to catalase and indoxyl acetate. Most of Campylobacter are susceptible to nalidixic acid and resistant to cephalothin (Barrett, Patton, & Morris, 1988). C. jejuni (subspecies jejuni) and C. coli are the most leading species among Campylobacter in public health concerns that cause gastroenteritis in human (WHO, 2016) and more likely to differentiate the two species with biochemical tests by using hydrolysis of hippurate (International Organization for Standardization [ISO], 2006). Other phenotypic characteristics such as oxidase positive, motility (81%), nitrate reduction, capability to grow on MacConkey’s agar, growth in 1% glycine, and unable to utilize glucose (Hunt, Abeyta, & Tran, 2001). In addition, the thermophilic C. jejuni requires temperature between 37 and 42 oC to grow (Davis & DiRita, 2008) with the optimum temperature at 41.5 oC (ISO, 2006). The bacteria are incapable to grow below 30 oC since cold