• Table one; shows the effect of water temperature has on the rate at which the anthocyanin pigment leaves of a beetroot tap root cells
• My groups results are highlighted in yellow and the class results are left blank
Temperature ͦC (+/- 1 ͦC) Amount of green light that was absorbed by the solution (+/- 0.001) Group 1 Group 2 Group 3 Group 4 Group 5 Group 6 Group 7
20 0.099 0.188 0.202 0.141 0.152 0.143 0.157
30 0.274 0.173 0.205 0.170 0.262 0.180 0.233
40 0.285 0.127 0.327 0.234 0.283 0.173 0.309
50 0.373 0.411 0.729 0.378 0.341 0.420 0.601
60 0.742 0.253 0.585 0.465 0.350 0.290 0.431
70 0.987 0.350 0.385 0.638 0.937 0.222 0.829
Wavelength (nm) 526.00 534.23 526.20 536.32 531.90 535.36 526.90
In beetroot cells the red anthocyanin pigment occurs in the vacuoles. Each vacuole is surrounded by a membrane and outside it the cytoplasm is surrounded by the plasma membrane. These membranes are too thin to be seen but we can draw certain conclusions about their properties.
Hypothesis; Membranes of the beetroot cells are composed of phospholipid bilayers. These form a stable fluid mosaic and retain their contents. The fluidity of the mosaic increases with temperature and high temperatures may result in the membrane becoming unstable. This would cause the anthocyanin pigment to leak …show more content…
Day one consisted of cutting the beetroots and placing them at each temperature while day two was used to measure how much green light was absorbed by the colorimeter. This could have affected the results because light could have reached the tubes causing some of the pigment to be damaged and not give accurate results. There are not many solutions for this but I suggest that we perform the lab experiment with sufficient time to do all of it in one day permitting more exact results on how much green light will be