Avian Flu Lab Report
Using an Eliza to test for Avian Flu
Methods
This experiment was performed in a plastic microtiter plate. Wells 1-12 of rows A and B were filled with 40 μL of stimulated antigen by using a micropipette. A clean pipette tip was used every time a new sample or reagent was added to the wells. Each sample was tested in triplicate and the amount of sample or reagent used was always 40 μL. The positive control were added to the first three wells of row A (A1, A2, A3), then the same amount of negative control was added to the next three wells (A4, A5,A6). Similarly, samples for Patient A, B, C, D, E, and F were added to wells A7-B12 as seen in Figure 1.
Figure 1: Sample microtiter plate used for this experiment. Legend: +C=> positive control, -C=>negative control, A-F =>patient samples A through F.
The stimulated secondary antibody was added to all the wells in rows A and B. Lastly, 40 μL of stimulated chromogen was added to all 24 wells (A1-12, B1-12). The plate was incubated at room temperature for 10 minutes. Observations were recorded.
Results
Figure 2: Microtiter plated after a 10 min incubation period at room temperature. The positive signs indicate the presence of the color purple in the wells after incubation. The intensity is displayed by the number of + sings present in each well. The highest intensity …show more content…
You do not show any sign of infection from the H1N5 virus. One of the chickens from the flock Patient B works with tested positive, however. Therefore, I would like to bring to you attention that transmission from ill poultry to humans is possible. I would recommend you limit and avoid contact with chickens that seem to be infected and wear a protective mask while working, since the virus often spreads through inhalation. The virus is not known to be transmitted from human to human unless they are in very close contact. Lastly, some other suggestion that might help in preventing future infections from
Methods
This experiment was performed in a plastic microtiter plate. Wells 1-12 of rows A and B were filled with 40 μL of stimulated antigen by using a micropipette. A clean pipette tip was used every time a new sample or reagent was added to the wells. Each sample was tested in triplicate and the amount of sample or reagent used was always 40 μL. The positive control were added to the first three wells of row A (A1, A2, A3), then the same amount of negative control was added to the next three wells (A4, A5,A6). Similarly, samples for Patient A, B, C, D, E, and F were added to wells A7-B12 as seen in Figure 1.
Figure 1: Sample microtiter plate used for this experiment. Legend: +C=> positive control, -C=>negative control, A-F =>patient samples A through F.
The stimulated secondary antibody was added to all the wells in rows A and B. Lastly, 40 μL of stimulated chromogen was added to all 24 wells (A1-12, B1-12). The plate was incubated at room temperature for 10 minutes. Observations were recorded.
Results
Figure 2: Microtiter plated after a 10 min incubation period at room temperature. The positive signs indicate the presence of the color purple in the wells after incubation. The intensity is displayed by the number of + sings present in each well. The highest intensity …show more content…
You do not show any sign of infection from the H1N5 virus. One of the chickens from the flock Patient B works with tested positive, however. Therefore, I would like to bring to you attention that transmission from ill poultry to humans is possible. I would recommend you limit and avoid contact with chickens that seem to be infected and wear a protective mask while working, since the virus often spreads through inhalation. The virus is not known to be transmitted from human to human unless they are in very close contact. Lastly, some other suggestion that might help in preventing future infections from