Atividade do Extrato de Própolis-Etanólico em Pacientes com Implantes Orais para diminuição de bactérias
The 2nd Kurdistan Conference on Biological Sciences
J. Duhok Univ. Vol.12, No.1 (Special Issue), Pp 233-239, 2009
University of Duhok 6-8 May, 2008
ANTIMICROBIAL ACTIVITY OF PROPOLIS COLLECTED IN DIFFERENT
REGIONS OF SULAIMANI PROVINCE-KURDISTAN REGION/IRAQ
HASTYAR H. NAJMADEEN and FARHAD A. KH. K AKAMAND
Dept of Biology, College of Science, University of Sulaimani, Kurdistan region, Iraq
(Accepted for publication: November 19, 2008)
ABSTRACT
The aim of this study was to investigate the activity of (28%) ethanolic extract propolis (EEP) of [Bazian, Pshdar, Sharbazher,
Khormal, Kanakawa and Sulaimani (city center)] against (Staphylococcus aureus, Staphylococcus epidermidis, Escherichia coli,
Pseudomonas aerugenosa, Klebsiella pneumoniae, Proteus mirabilis and yeast Candida albicans). Two methods were employed for propolis activity evaluation; firstly by agar well diffusion method using 40µl of propolis extracts and ethanol 70% as the solvent control per well against tested bacteria and fungus and more commonly used antibiotics were used in comparison to propolis samples. The second method was employed by measuring Minimal Inhibitory Concentration & Minimal Bactericidal; Fungicidal
Concentrations (MIC, MBC & MFC) using two fold dilution method for propolis extracts and ethanol 70% as a solvent control in serial concentrations. The Results exhibited that propolis is more efficient than some antibiotic discs. The most sensitive bacteria against (EEP) with mean inhibitory diameters (22-26) mm was S. epidermidis followed by S. aerues and C. albicans (15-22) mm.
Tested Gram negative bacteria have lowest inhibitory zones (7-9) mm. Results of MIC & MBC showed S. aureus with (0.1750.7mg/ml) followed by S. epidermidis & C. albicans with (0.7-1.4mg/ml). Among tested Gram negative bacteria, all (EEP) samples showed relatively strong inhibitory action on P. mirabilis (14mg/ml) and weak inhibitory action on P. aerugenosa & K. pneumoniae
(28mg/ml) of
J. Duhok Univ. Vol.12, No.1 (Special Issue), Pp 233-239, 2009
University of Duhok 6-8 May, 2008
ANTIMICROBIAL ACTIVITY OF PROPOLIS COLLECTED IN DIFFERENT
REGIONS OF SULAIMANI PROVINCE-KURDISTAN REGION/IRAQ
HASTYAR H. NAJMADEEN and FARHAD A. KH. K AKAMAND
Dept of Biology, College of Science, University of Sulaimani, Kurdistan region, Iraq
(Accepted for publication: November 19, 2008)
ABSTRACT
The aim of this study was to investigate the activity of (28%) ethanolic extract propolis (EEP) of [Bazian, Pshdar, Sharbazher,
Khormal, Kanakawa and Sulaimani (city center)] against (Staphylococcus aureus, Staphylococcus epidermidis, Escherichia coli,
Pseudomonas aerugenosa, Klebsiella pneumoniae, Proteus mirabilis and yeast Candida albicans). Two methods were employed for propolis activity evaluation; firstly by agar well diffusion method using 40µl of propolis extracts and ethanol 70% as the solvent control per well against tested bacteria and fungus and more commonly used antibiotics were used in comparison to propolis samples. The second method was employed by measuring Minimal Inhibitory Concentration & Minimal Bactericidal; Fungicidal
Concentrations (MIC, MBC & MFC) using two fold dilution method for propolis extracts and ethanol 70% as a solvent control in serial concentrations. The Results exhibited that propolis is more efficient than some antibiotic discs. The most sensitive bacteria against (EEP) with mean inhibitory diameters (22-26) mm was S. epidermidis followed by S. aerues and C. albicans (15-22) mm.
Tested Gram negative bacteria have lowest inhibitory zones (7-9) mm. Results of MIC & MBC showed S. aureus with (0.1750.7mg/ml) followed by S. epidermidis & C. albicans with (0.7-1.4mg/ml). Among tested Gram negative bacteria, all (EEP) samples showed relatively strong inhibitory action on P. mirabilis (14mg/ml) and weak inhibitory action on P. aerugenosa & K. pneumoniae
(28mg/ml) of
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