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Name: Madison Gullatt Sect/Table: !/D
Experiment 8 Report: Extracellular Enzymes
Purpose:
The purpose of this experiment is to perform a variety of different test and record the data from each test in relation to extracellular enzymes.
Results:
1. Fill in the following tables with your observations. Note if the test is positive or negative for the enzyme. Also note the appearance of the colonies and/or media. This will include: the color of the medium immediately surrounding the colonies after addition of iodine on PDA plate, whether bubbles were observed or not in the catalase test, the color of the urea slant, the color of the citrate slant, and whether the rabbit plasma remained liquid or became solid due to clot formation, etc. After each observation note which results indicate production of the given enzymes and which results indicate non-production of the given enzyme by placing a “+” or “-“in the appropriate squares.
A. Starch Hydrolysis
Note: the color of the medium immediately surrounding the colonies after addition of iodine on PDA plate.
Organism
Result
+ or -
Appearance/Observations
Bacillus cereus
Escherichia coli
B. Gelatin Hydrolysis (gelatinase production)
Note: whether the gelatin deep remains solid or has been liquefied
Organism
Result
+ or -
Appearance/Observations
Bacillus subtilis
Escherichia coli
C. Coagualse: identification of Staphylococcus aureus
Note: whether the rabbit plasma has remained liquid or become solid due to clot formation
Organism
Result
+ or -
Appearance/Observations
Staphylococcus aureus
Staphylococcous epidermidis
D. Urease Acitvity: identification of Proteus spp.
Note: the color of the urease slant
Organism
Result
+ or -
Appearance/Observations
Proteus vulgaris
+
Bright pink in color
Escherichia coli
-
Yellow in color
E. Hemolysins
Note: the appearance of the colonies and/or media.
Organism
Result
Experiment 8 Report: Extracellular Enzymes
Purpose:
The purpose of this experiment is to perform a variety of different test and record the data from each test in relation to extracellular enzymes.
Results:
1. Fill in the following tables with your observations. Note if the test is positive or negative for the enzyme. Also note the appearance of the colonies and/or media. This will include: the color of the medium immediately surrounding the colonies after addition of iodine on PDA plate, whether bubbles were observed or not in the catalase test, the color of the urea slant, the color of the citrate slant, and whether the rabbit plasma remained liquid or became solid due to clot formation, etc. After each observation note which results indicate production of the given enzymes and which results indicate non-production of the given enzyme by placing a “+” or “-“in the appropriate squares.
A. Starch Hydrolysis
Note: the color of the medium immediately surrounding the colonies after addition of iodine on PDA plate.
Organism
Result
+ or -
Appearance/Observations
Bacillus cereus
Escherichia coli
B. Gelatin Hydrolysis (gelatinase production)
Note: whether the gelatin deep remains solid or has been liquefied
Organism
Result
+ or -
Appearance/Observations
Bacillus subtilis
Escherichia coli
C. Coagualse: identification of Staphylococcus aureus
Note: whether the rabbit plasma has remained liquid or become solid due to clot formation
Organism
Result
+ or -
Appearance/Observations
Staphylococcus aureus
Staphylococcous epidermidis
D. Urease Acitvity: identification of Proteus spp.
Note: the color of the urease slant
Organism
Result
+ or -
Appearance/Observations
Proteus vulgaris
+
Bright pink in color
Escherichia coli
-
Yellow in color
E. Hemolysins
Note: the appearance of the colonies and/or media.
Organism
Result