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Ap Biology Lab Design Lab

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Ap Biology Lab Design Lab
Biology 20 IB Design Lab
Does increasing or decreasing the ph level of a 3% concentration H2O2 (l) solution affect the amount of oxygen released between 15.0ml of the solution and a 5g sample of liver? The reaction is measured by the difference in mass of a balloon that is used to capture the amount of gas released by the catalase enzyme found in liver reacting with a basified 3% H2O2 (l) and an acidified 3% H2O2 (l) .

Chemical reactions occur when two or more molecules interact and the molecules change. Bonds between atoms are broken and recreated to make new molecules. Enzymes are large proteins that speed up the rate of a chemical reaction by acting as a catalyst and remain unchanged,they are natural biochemical catalyst. Certain catalysts work
…show more content…
Using the same weigh scale that was used for measuring the mass of the liver, weigh out 6 15.0g (15.0ml) samples of a 3% H2O2 (l) solution in test tubes.
Using the same scale as before, weigh out 3 3.0g samples of baking soda on parchment paper and 3 3.0g samples of vinegar in 3 beakers.
Mix the 3 samples of Baking soda with 3 of the samples of H2O2 (l) and mix the 3 samples of vinegar with the other sample of baking soda.
Put rubber stoppers on the test tubes and mix/stir all 6 solutions. Be cautious of the Baking soda solution as they may produce CO2 (g) when shaken so be cautious of the stopper blowing off the top of the test tube.
Using 6 strips of pH paper, test the pH levels all solutions to ensure that there was a change in the acidity and basicity of the solutions. The solution with baking soda should be around 8 and the solutions that were mixed with vinegar should have a pH of around 5.
Weigh the initial mass of 6 balloons using the same scale as before.
Pour the altered solution into the six balloons making sure to label each balloon as either acidic or basic.
Attach the balloons to the six erlenmeyer flask containing the liver

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