Anaphase Promoting Complex (APC)
Chapter 15 Signal Transduction
1) Endocrine, paracrine , autocrine signaling, and cell-cell contact (Fig. 15-2). Endocrine signaling is long distance signaling. An example would be pancreatic cells secreting insulin. Paracrine signaling is for close proximity. An example would be a nerve cell releasing neurotransmitters. In autocrine signaling the cell that produces the ligand also contains the receptor for that ligand. This is how cancer cells work. In signaling by plasma membrane attached proteins, the target cell does something in response to direct contact from the signaling cell.
2) List examples of 1) steroid hormones and 2) amino acid derivatives that act as ligands. What are the catecholamines, and which amino acid are …show more content…
It is comprised of Cdk1-Cyclin B * The name came from studying frog oocyte maturation in vitro
9) The pathway to MPF destruction: What is Anaphase Promoting Complex (APC)? What activates APC? What does APC then do and how does it do it? Fig. 20-10 * MPF is a kinase that phosphorylates many different substrates to initiate mitotic events * To exit mitosis, MPF must be destroyed * Destruction of MPF depends on the destruction of Cyclin B * Destruction of Cyclin B is via the ubiquitin pathway * Ubiquitin is covalently linked to lysines behind the destruction box * Cyclin B without the destruction box will not be destroyed * APC destroys MPF, but APC was actually activated earlier at anaphase by MPF
10) You have to know Fig. 20-13 and 20-14!!
11) Molecular events at the onset of mitosis:
a) Nuclear envelope disassembly: what are the lamin proteins, how do they disassemble, and where do they go when they disassemble? (Figs. 20-16, 20-17) * The nuclear lamina supports the nuclear envelope. It is found on the underside of the inner envelope membrane. * The nuclear lamina is made of three lamin proteins: A, B and
1) Endocrine, paracrine , autocrine signaling, and cell-cell contact (Fig. 15-2). Endocrine signaling is long distance signaling. An example would be pancreatic cells secreting insulin. Paracrine signaling is for close proximity. An example would be a nerve cell releasing neurotransmitters. In autocrine signaling the cell that produces the ligand also contains the receptor for that ligand. This is how cancer cells work. In signaling by plasma membrane attached proteins, the target cell does something in response to direct contact from the signaling cell.
2) List examples of 1) steroid hormones and 2) amino acid derivatives that act as ligands. What are the catecholamines, and which amino acid are …show more content…
It is comprised of Cdk1-Cyclin B * The name came from studying frog oocyte maturation in vitro
9) The pathway to MPF destruction: What is Anaphase Promoting Complex (APC)? What activates APC? What does APC then do and how does it do it? Fig. 20-10 * MPF is a kinase that phosphorylates many different substrates to initiate mitotic events * To exit mitosis, MPF must be destroyed * Destruction of MPF depends on the destruction of Cyclin B * Destruction of Cyclin B is via the ubiquitin pathway * Ubiquitin is covalently linked to lysines behind the destruction box * Cyclin B without the destruction box will not be destroyed * APC destroys MPF, but APC was actually activated earlier at anaphase by MPF
10) You have to know Fig. 20-13 and 20-14!!
11) Molecular events at the onset of mitosis:
a) Nuclear envelope disassembly: what are the lamin proteins, how do they disassemble, and where do they go when they disassemble? (Figs. 20-16, 20-17) * The nuclear lamina supports the nuclear envelope. It is found on the underside of the inner envelope membrane. * The nuclear lamina is made of three lamin proteins: A, B and